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Titolo:
ISOLATION AND PROPERTIES OF A SIALOGLYCOPROTEIN FROM THE SKIN MUCUS OF THE STINGRAY DASYATIS-AKAJEI
Autore:
SUMI T; HAMA Y; MARUYAMA D; ASAKAWA M; NAKAGAWA H;
Indirizzi:
SAGA UNIV,FAC AGR,DEPT APPL BIOL SCI SAGA 840 JAPAN SAGA UNIV,FAC AGR,DEPT APPL BIOL SCI SAGA 840 JAPAN KUMAMOTO UNIV,FAC EDUC,FOOD SCI LAB KUMAMOTO 860 JAPAN
Titolo Testata:
Fisheries science
fascicolo: 3, volume: 63, anno: 1997,
pagine: 453 - 458
SICI:
0919-9268(1997)63:3<453:IAPOAS>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
GLYCOPROTEINS; PURIFICATION;
Keywords:
SIALOGLYCOPROTEIN; MUCIN; MUCUS GLYCOPROTEIN; MUCUS; SIALIC ACID; STINGRAY; DASYATIS AKAJEI;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Science Citation Index Expanded
Citazioni:
22
Recensione:
Indirizzi per estratti:
Citazione:
T. Sumi et al., "ISOLATION AND PROPERTIES OF A SIALOGLYCOPROTEIN FROM THE SKIN MUCUS OF THE STINGRAY DASYATIS-AKAJEI", Fisheries science, 63(3), 1997, pp. 453-458

Abstract

A sialoglycoprotein (SGP) has been purified from the skin mucus of the stingray Dasyatis akajei. SGP contained 22.1% (w/w) NeuAc, 24.4% GalNAc, 9.9% GlcNAc, 6.1% Gal, and 27% amino acids, and its average molecular weight (Mr) was estimated to be 500,000. SGP was very rich in Thr(32 mol%) and Ser (12 mol%). Treatment of SGP with alkali (beta-elimination of carbohydrate chains) resulted in the destruction of 70% of Thr and Ser, indicating that the carbohydrate chains were attached through these amino acids. Exhaustive digestion of SGP by actinase yieldeda highly glycosylated glycopeptide with Mr of 50,000. Based on these results, it is assumed that a SGP molecule bears more than 400 oligosaccharide chains which are attached to the Thr and Ser residues of the polypeptide backbone and spaced at an average of 3 amino acids apart.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 22/01/20 alle ore 07:29:52