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Titolo:
Visualization of fibrillar amyloid deposits in living, transgenic Caenorhabditis elegans animals using the sensitive amyloid dye, X-34
Autore:
Link, CD; Johnson, CJ; Fonte, V; Paupard, MC; Hall, DH; Styren, S; Mathis, CA; Klunk, WE;
Indirizzi:
Univ Colorado, Inst Behav Genet, Boulder, CO 80309 USA Univ Colorado Boulder CO USA 80309 nst Behav Genet, Boulder, CO 80309 USA Yeshiva Univ Albert Einstein Coll Med, Dept Neurosci, Bronx, NY 10461 USA Yeshiva Univ Albert Einstein Coll Med Bronx NY USA 10461 nx, NY 10461 USA Aventis Pharmaceut, Bridgewater, NJ 08807 USA Aventis Pharmaceut Bridgewater NJ USA 08807 ut, Bridgewater, NJ 08807 USA Univ Pittsburgh, Sch Med, Dept Radiol, PET Facil, Pittsburgh, PA 15213 USAUniv Pittsburgh Pittsburgh PA USA 15213 T Facil, Pittsburgh, PA 15213 USA Univ Pittsburgh, Sch Med, Dept Psychiat, Pittsburgh, PA 15213 USA Univ Pittsburgh Pittsburgh PA USA 15213 sychiat, Pittsburgh, PA 15213 USA
Titolo Testata:
NEUROBIOLOGY OF AGING
fascicolo: 2, volume: 22, anno: 2001,
pagine: 217 - 226
SICI:
0197-4580(200103/04)22:2<217:VOFADI>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
ELECTRON-MICROSCOPY; ALZHEIMERS-DISEASE; CONGO RED; PROTEIN; TISSUE; NEUROTOXICITY; ACCUMULATION; EXPRESSION; SECTIONS; PEPTIDE;
Keywords:
abeta; amyloid; C. elegans; transgenic; X-34; in vivo staining; immuno-EM; fibrillar ultrastructure; transthyretin; 4G8; BSB;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
24
Recensione:
Indirizzi per estratti:
Indirizzo: Link, CD Univ Colorado, Inst Behav Genet, Campus Box 447, Boulder, CO 80309 USA Univ Colorado Campus Box 447 Boulder CO USA 80309 r, CO 80309 USA
Citazione:
C.D. Link et al., "Visualization of fibrillar amyloid deposits in living, transgenic Caenorhabditis elegans animals using the sensitive amyloid dye, X-34", NEUROBIOL A, 22(2), 2001, pp. 217-226

Abstract

Transgenic Caenorhabditis elegans animals can be engineered to express high levels of the human beta amyloid peptide (Abeta). Histochemistry of fixedtissue from these animals reveals deposits reactive with the amyloid-specific dyes Congo Red and thioflavin S (Fay et al., J. Neurochem 71:1616, 1998). Here we show by immune-electron microscopy that these animals contain intracellular immunoreactive deposits with classic amyloid fibrillar ultrastructure. These deposits can be visualized in living animals using the newly developed, intensively fluorescent, amyloid-specific dye X-34. This in vivostaining allows monitoring of amyloid deposition in individual animals over time. The specificity of this staining is demonstrated by examining transgenic animals expressing high levels of a non-fibrillar beta peptide variant, the beta single-chain dimer, These animals have deposits immunoreactive with anti-beta antibodies, but do not have X-34 deposits or deposits with afibrillar ultrastructure. X-34 can also be used in vivo to visualize putative amyloid deposits resulting from accumulation of human transthyretin, another amyloidic protein. In vivo amyloid staining with X-34 may be a usefultool for monitoring anti-amyloidic treatments in real time or screening for genetic alterations that affect amyloid formation. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 23:13:15