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Titolo:
Calcium accelerates endocytosis of vSNAREs at hippocampal synapses
Autore:
Sankaranarayanan, S; Ryan, TA;
Indirizzi:
Cornell Univ, Weill Med Coll, Dept Biochem, New York, NY 10021 USA CornellUniv New York NY USA 10021 , Dept Biochem, New York, NY 10021 USA
Titolo Testata:
NATURE NEUROSCIENCE
fascicolo: 2, volume: 4, anno: 2001,
pagine: 129 - 136
SICI:
1097-6256(200102)4:2<129:CAEOVA>2.0.ZU;2-Y
Fonte:
ISI
Lingua:
ENG
Soggetto:
FROG NEUROMUSCULAR-JUNCTION; SYNAPTIC VESICLE MEMBRANE; ADRENAL CHROMAFFIN CELLS; TRANSMITTER RELEASE; RAPID ENDOCYTOSIS; V-ATPASES; DYNAMIN; TRANSMISSION; EXOCYTOSIS; INHIBITION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
28
Recensione:
Indirizzi per estratti:
Indirizzo: Ryan, TA Cornell Univ, Weill Med Coll, Dept Biochem, 1300 York Ave, New York, NY 10021 USA Cornell Univ 1300 York Ave New York NY USA 10021 rk, NY 10021 USA
Citazione:
S. Sankaranarayanan e T.A. Ryan, "Calcium accelerates endocytosis of vSNAREs at hippocampal synapses", NAT NEUROSC, 4(2), 2001, pp. 129-136

Abstract

A pH-sensitive form of green-fluorescent protein (GFP) fused to the lumenal domain of VAMP (synapto-pHluorin) provides a sensitive optical probe to track the net balance between exocytosis and endocytosis of this protein at small synaptic terminals of the central nervous system. Here we used a reversible proton-pump blocker that prevents vesicle re-acidification upon endocytosis to trap vesicles in the alkaline state during recycling. In combination with optical measurements of synapto-pHluorin, we used alkaline trapping to examine the kinetic components of exocytosis and endocytosis separately at synaptic terminals. Using this approach, we show that, in addition tocontrolling exocytosis, intracellular calcium levels tightly regulate the speed of endocytosis, increasing it to a maximal speed of approximately onevesicle per second.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/06/20 alle ore 02:07:31