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Titolo:
Polo-like kinase 1 phosphorylates cyclin B1 and targets it to the nucleus during prophase
Autore:
Toyoshima-Morimoto, F; Taniguchi, E; Shinya, N; Iwamatsu, A; Nishida, E;
Indirizzi:
Kyoto Univ, Grad Sch Sci, Dept Biophys, Sakyo Ku, Kyoto 6068502, Japan Kyoto Univ Kyoto Japan 6068502 t Biophys, Sakyo Ku, Kyoto 6068502, Japan Kyoto Univ, Grad Sch Biostudies, Dept Cell & Dev Biol, Sakyo Ku, Kyoto 6068502, Japan Kyoto Univ Kyoto Japan 6068502 Dev Biol, Sakyo Ku, Kyoto 6068502, Japan Kirin Brewery Co Ltd, Cent Labs Key Technol, Kanazawa Ku, Yokohama, Kanagawa 2360004, Japan Kirin Brewery Co Ltd Yokohama Kanagawa Japan 2360004 agawa 2360004, Japan
Titolo Testata:
NATURE
fascicolo: 6825, volume: 410, anno: 2001,
pagine: 215 - 220
SICI:
0028-0836(20010308)410:6825<215:PK1PCB>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
CELL-CYCLE; SUBCELLULAR-LOCALIZATION; DNA-DAMAGE; M-PHASE; EXPORT; MITOSIS; SIGNAL; PLX1; TRANSPORT; CRM1;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Physical, Chemical & Earth Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Nishida, E Kyoto Univ, Grad Sch Sci, Dept Biophys, Sakyo Ku, Kyoto 6068502, Japan Kyoto Univ Kyoto Japan 6068502 Sakyo Ku, Kyoto 6068502, Japan
Citazione:
F. Toyoshima-Morimoto et al., "Polo-like kinase 1 phosphorylates cyclin B1 and targets it to the nucleus during prophase", NATURE, 410(6825), 2001, pp. 215-220

Abstract

In vertebrate cells, the nuclear entry of Cdc2-cyclin B1 (MPF1-3) during prophase(4-6) is thought to be essential for the induction and coordination of M-phase events(7-12). Phosphorylation of cyclin B1 is central to its nuclear translocation(8,13,14), but the kinases that are responsible remain unknown. Here we have purified a protein kinase from Xenopus M-phase extractsthat phosphorylates a crucial serine residue (S147) in the middle of the nuclear export signal sequence(10,13,15) of cyclin B1. We have identified this kinase as Plx1 (ref. 16), a Xenopus homologue of Polo-like kinase (Plk)-1 (refs 17, 18). During cell-cycle progression in HeLa cells, a change in the kinase activity of endogenous Plk1 toward S147 and/or S133 correlates with a kinase activity in the cell extracts. An anti-Plk1 antibody depletes the M-phase extracts of the kinase activity toward S147 and/or S133. An anti-phospho-S147 antibody reacts specifically with cyclin B1 only during G2/M phase. A mutant cyclin B1 in which S133 and S147 are replaced by alanines remains in the cytoplasm, whereas wild-type cyclin B1 accumulates in the nucleus during prophase. Co-expression of constitutively active Plk1 stimulates nuclear entry of cyclin B1. Our results indicate that Plk1 may be involved in targeting MPF to the nucleus during prophase.

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Documento generato il 20/01/21 alle ore 03:07:27