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Titolo:
Regulation of in vitro penetration of frozen-thawed boar spermatozoa by caffeine and adenosine
Autore:
Funahashi, H; Nagai, T;
Indirizzi:
Okayama Univ, Fac Agr, Dept Anim Sci, Okayama 7008530, Japan Okayama UnivOkayama Japan 7008530 Dept Anim Sci, Okayama 7008530, Japan Tohoku Natl Agr Expt Stn, Morioka, Iwate, Japan Tohoku Natl Agr Expt Stn Morioka Iwate Japan Stn, Morioka, Iwate, Japan
Titolo Testata:
MOLECULAR REPRODUCTION AND DEVELOPMENT
fascicolo: 4, volume: 58, anno: 2001,
pagine: 424 - 431
SICI:
1040-452X(200104)58:4<424:ROIVPO>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
MATURED PORCINE OOCYTES; FERTILIZATION IN-VITRO; PIG OOCYTES; INVITRO FERTILIZATION; DEVELOPMENTAL COMPETENCE; ADENYLATE-CYCLASE; ACROSOME REACTION; ZONA-PELLUCIDA; MATURATION; CAPACITATION;
Keywords:
caffeine; adenosine; in vitro fertilization; polyspermy; porcine oocytes;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
35
Recensione:
Indirizzi per estratti:
Indirizzo: Funahashi, H Okayama Univ, Fac Agr, Dept Anim Sci, 1-1-1 Tsushima Naka, Okayama 7008530, Japan Okayama Univ 1-1-1 Tsushima Naka Okayama Japan 7008530 Japan
Citazione:
H. Funahashi e T. Nagai, "Regulation of in vitro penetration of frozen-thawed boar spermatozoa by caffeine and adenosine", MOL REPROD, 58(4), 2001, pp. 424-431

Abstract

Effects of caffeine and adenosine on the function and in vitro penetrationof frozen-thawed boar spermatozoa were examined. First, the effect on sperm function was determined by the chlortetracycline fluorescence assessment. Both caffeine and adenosine stimulated capacitation of spermatozoa. However, adenosine, but not caffeine, inhibited spontaneous acrosome loss. Second, sperm penetration into in vitro matured oocytes was compared among spermatozoa cultured in the absence or presence of caffeine or adenosine. Both caffeine and adenosine increased the penetration rate (99.1 +/- 0.9% in caffeine, 72.4 +/- 2.0% in adenosine vs. 54.8 +/- 5.1% in controls) but only caffeine decreased drastically the monospermic penetration rate (8.0 +/- 2.3% in caffeine vs. 75.4 +/- 4.8% in adenosine and 78.6 +/- 4.8% in controls). When oocytes were cocultured in various sperm concentrations, the proportion of monospermy changed in inverse proportion to sperm concentration in thepresence of caffeine, but did not change in the presence of adenosine. A relatively high number of spermatozoa at the early stage of spontaneous acrosome reaction in the presence of caffeine may be one of the main causes of polyspermic penetration in porcine IVF system. These results indicate that replacement of caffeine with adenosine in fertilization medium improves monospermic penetration by frozen-thawed boar spermatozoa. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/08/20 alle ore 00:50:48