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Titolo:
In vitro reproductive toxicity of polychlorinated biphenyls: Effects on oocyte maturation and developmental competence in cattle
Autore:
Pocar, P; Perazzoli, F; Luciano, AM; Gandolfi, F;
Indirizzi:
Univ Milan, Dept Anat & Domest Anim, Milan, Italy Univ Milan Milan Italy niv Milan, Dept Anat & Domest Anim, Milan, Italy
Titolo Testata:
MOLECULAR REPRODUCTION AND DEVELOPMENT
fascicolo: 4, volume: 58, anno: 2001,
pagine: 411 - 416
SICI:
1040-452X(200104)58:4<411:IVRTOP>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
IN-VITRO; ORGANOCHLORINE PESTICIDES; BOVINE OOCYTES; GOLDEN-HAMSTER; FERTILIZATION; FISH; CONSUMPTION; CHEMICALS; EMBRYOS; CALCIUM;
Keywords:
polychlorinated biphenyls (PCBs); endocrine disruptive compounds (EDC); IVM; IVF; embryo development;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
31
Recensione:
Indirizzi per estratti:
Indirizzo: Gandolfi, F Ist Anat Anim Domest, Via Trentacoste 2, I-20134 Milan, Italy Ist Anat Anim Domest Via Trentacoste 2 Milan Italy I-20134 ly
Citazione:
P. Pocar et al., "In vitro reproductive toxicity of polychlorinated biphenyls: Effects on oocyte maturation and developmental competence in cattle", MOL REPROD, 58(4), 2001, pp. 411-416

Abstract

Polychlorinated biphenyls (PCBs) are one of the most persistent and widespread group of endocrine disrupting compounds in the ecosystem. High concentrations of these substances are known to be present in sewage sludge from industrial, agricultural, and domestic origin that is spread in increasing amounts on arable land and pasture as fertilizer and is found in water, representing an increasing risk for the reproductive health of farm animals. Objective of this study was to determine the impact of PCBs on maturation anddevelopmental competence of cattle oocytes. Since PCBs are a family of 209molecules present in the environ ment as a mixture, Aroclor-12 54, a pool of more than 60 congeners, was used in these experiments as its compositionis considered to be environmentally relevant. Cumulus-oocytes complexes were exposed during IVM to serial concentrations of Aroclor-1254 (between 1 mug/ml and 0.0001 mug/ml) and compared with control groups. Aroclor decreased the percentage of oocytes that reached metaphase ii stage after 24 hr, atdoses as law as 0.01 mug/ml. Groups treated with 0.001 mug/ml or above, showed an impaired fertilization rate and a dramatic increase of polyspermy. Moreover, exposure during maturation resulted in a reduced proportion of oocytes that cleaved and developed until blastocyst stage although no differences in embryo cell numbers were observed. The present study indicates thatvery low PCBs concentrations are sufficient to disrupt bovine oocyte maturation, its fertilization, and developmental competence. These results also provide a set of reference data for the assessment of the risk posed by these substances to animal reproductive health, though further work wi II be necessary to equate in vitro doses to in vive exposures. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/12/20 alle ore 13:12:19