Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Alterations in potassium channel gene expression in atria of patients withpersistent and paroxysmal atrial fibrillation: Differential regulation of protein and mRNA levels for K+ channels
Autore:
Brundel, BJJM; Van Gelder, IC; Henning, RH; Tuinenburg, AE; Wietses, M; Grandjean, JG; Wilde, AAM; Van Gilst, WH; Crijns, HJGM;
Indirizzi:
Univ Groningen Hosp, Thoraxctr, Dept Cardiol, NL-9700 RB Groningen, Netherlands Univ Groningen Hosp Groningen Netherlands NL-9700 RB ningen, Netherlands Univ Groningen Hosp, Thoraxctr, Dept Clin Pharmacol, NL-9700 RB Groningen,Netherlands Univ Groningen Hosp Groningen Netherlands NL-9700 RB oningen,Netherlands Univ Groningen Hosp, Thoraxctr, Dept Thorac Surg, NL-9700 RB Groningen, Netherlands Univ Groningen Hosp Groningen Netherlands NL-9700 RB ningen, Netherlands Univ Amsterdam, Dept Cardiol, NL-1012 WX Amsterdam, Netherlands Univ Amsterdam Amsterdam Netherlands NL-1012 WX X Amsterdam, Netherlands
Titolo Testata:
JOURNAL OF THE AMERICAN COLLEGE OF CARDIOLOGY
fascicolo: 3, volume: 37, anno: 2001,
pagine: 926 - 932
SICI:
0735-1097(20010301)37:3<926:AIPCGE>2.0.ZU;2-8
Fonte:
ISI
Lingua:
ENG
Soggetto:
DELAYED RECTIFIER CURRENT; ELECTROPHYSIOLOGICAL CHARACTERISTICS; MYOCARDIAL-ISCHEMIA; OUTWARD CURRENT; CROSS-LINKING; DOG-MODEL; MECHANISMS; CARDIOMYOCYTES; VERAPAMIL; MYOCYTES;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Van Gelder, IC Univ Groningen Hosp, Thoraxctr, Dept Cardiol, POB 30001, NL-9700 RB Groningen, Netherlands Univ Groningen Hosp POB 30001 Groningen Netherlands NL-9700 RB
Citazione:
B.J.J.M. Brundel et al., "Alterations in potassium channel gene expression in atria of patients withpersistent and paroxysmal atrial fibrillation: Differential regulation of protein and mRNA levels for K+ channels", J AM COL C, 37(3), 2001, pp. 926-932

Abstract

OBJECTIVES Our purpose was to determine whether patients with persistent atrial fibrillation (AF) and patients with paroxysmal AF show alterations inpotassium channel expression. BACKGROUND Persistent AF is associated with a sustained shortening of the atrial action potential duration and atrial refractory period. Underlying molecular changes have not been studied in humans. We, investigated whether a changed gene expression of specific potassium channels is associated withthese changes in patients with persistent AF and in patients with paroxysmal AF. METHODS Right atrial appendages were obtained from 8 patients with paroxysmal AF, 10 with persistent AF and 18 matched controls in sinus rhythm. All controls underwent coronary artery bypass surgery, whereas most AF patientsunderwent Cox's MAZE surgery (atrial arrhythmia surgery to cure AF) (n = 12). All patients had normal left ventricular function. mRNA (ribonucleic acid) levels were measured by semiquantitative polymerase chain reaction and protein content by Western blotting. RESULTS mRNA levels of transient outward channel (Kv4.3), acetylcholine-dependent potassium channel (Kir3.4) and ATP-dependent potassium channel (Kir6.2) were reduced in patients with persistent AF (-35%, -47%, and -36%, respectively, p < 0.05), whereas only Kv4.3 mRNA level was reduced in patientswith paroxysmal AF (-29%, p = 0.03). No changes were found for Kv1.5 and HERG mRNA levels in either group. Protein levels of Kv4.3, Kv1.5 and Kir3.1 were reduced both in patients with persistent AF (-39%, -84% and -47%, respectively, p < 0.05) and in those with paroxysmal AF (-57%, -64%, and -40%, respectively, p < 0.05). CONCLUSIONS Persistent AF is accompanied by reductions in mRNA and proteinlevels of several potassium channels. In patients with paroxysmal AF thesereductions were observed predominantly at the protein level and not at themRNA level, suggesting a post-transcriptional regulation. (J Am Coil Cardiol 2001;37:926-32) (C) 2001 by the American College of Cardiology.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 12:43:00