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Titolo:
Osmotic pretreatment promotes axillary shooting from cauliflower curd pieces by acting through internal cytokinin level modifications
Autore:
Vandemoortele, JL; Kevers, C; Billard, JP; Gaspar, T;
Indirizzi:
SILEBAN, Stn Expt Gatteville, F-50760 Barfleur, France SILEBAN Barfleur France F-50760 xpt Gatteville, F-50760 Barfleur, France Univ Caen, IRBA, Lab Physiol & Biochim Vegetales, F-14032 Caen, France Univ Caen Caen France F-14032 & Biochim Vegetales, F-14032 Caen, France Univ Liege, Inst Bot B22, B-4000 Liege, Belgium Univ Liege Liege BelgiumB-4000 ege, Inst Bot B22, B-4000 Liege, Belgium
Titolo Testata:
JOURNAL OF PLANT PHYSIOLOGY
fascicolo: 2, volume: 158, anno: 2001,
pagine: 221 - 225
SICI:
0176-1617(200102)158:2<221:OPPASF>2.0.ZU;2-N
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENDOGENOUS CYTOKININS; CULTURE-MEDIUM; BENZYLADENINE; VITRIFICATION; INVITRO;
Keywords:
acclimatization; Brassica oleracea L. var botrytis; micropropagation; sucrose; zeatin; zeatin riboside;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Vandemoortele, JL SILEBAN, Stn Expt Gatteville, F-50760 Barfleur, France SILEBAN Barfleur France F-50760 50760 Barfleur, France
Citazione:
J.L. Vandemoortele et al., "Osmotic pretreatment promotes axillary shooting from cauliflower curd pieces by acting through internal cytokinin level modifications", J PLANT PHY, 158(2), 2001, pp. 221-225

Abstract

In vitro propagation of cauliflower has generally been achieved through axillary shoot proliferation of curd explants on Murashige and Skoog (MS) medium supplemented with an auxin and a cytokinin. Recently, it has been shown(Vandemoortele 1999) that a soaking in sucrose (-2 MPa for 24 h) of cauliflower curd explants, before culture without any growth regulator, also induced axillary branching. The later procedure avoids the phenomenon of hyperhydricity in the shoots formed. Axillary shooting obtained by the two methods appears to be mediated by modifications of internal cytokinin levels. Theosmotic pretreatment did not influence auxin levels, but induced a zeatin and a zeatin riboside levels increase. Curd explants cultured with the usual procedure (on MS medium supplemented with 5 mu mol/L BA and 0.5 mu mol/L NAA) showed a zeatin and zeatin riboside levels increase of the same magnitude and a higher one for isopentenyl adenine and isopentenyl adenosine. Themodification of the cytokinin status in the curd explants subjected to a short osmotic pretreatment thus should be less favourable for hyperhydricity.

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Documento generato il 25/09/20 alle ore 06:57:19