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Titolo:
Probing the link between proton transport and water content in lipid membranes
Autore:
Krishnamoorthy, I; Krishnamoorthy, G;
Indirizzi:
Tata Inst Fundamental Res, Dept Chem Sci, Bombay 400005, Maharashtra, India Tata Inst Fundamental Res Bombay Maharashtra India 400005 arashtra, India
Titolo Testata:
JOURNAL OF PHYSICAL CHEMISTRY B
fascicolo: 7, volume: 105, anno: 2001,
pagine: 1484 - 1488
SICI:
1520-6106(20010222)105:7<1484:PTLBPT>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
RESOLVED FLUORESCENCE MICROSCOPY; MOLECULAR-DYNAMICS SIMULATION; MAXIMUM-ENTROPY METHOD; CELL PLASMA-MEMBRANES; GPI-ANCHORED PROTEINS; PHOSPHOLIPID-BILAYERS; PHYSICAL STATE; CHOLESTEROL; PERMEABILITY; FLUIDITY;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
60
Recensione:
Indirizzi per estratti:
Indirizzo: Krishnamoorthy, I Tata Inst Fundamental Res, Dept Chem Sci, Homi Bhabha Rd, Bombay 400005, Maharashtra, India Tata Inst Fundamental Res Homi Bhabha Rd Bombay Maharashtra India 400005
Citazione:
I. Krishnamoorthy e G. Krishnamoorthy, "Probing the link between proton transport and water content in lipid membranes", J PHYS CH B, 105(7), 2001, pp. 1484-1488

Abstract

The rate of transport of protons across lipid membranes is anomalously high when compared to that of other monovalent cations. The H-bonded water-wire mechanism had been proposed earlier for explaining this anomaly. We have probed the dynamics of lipid membranes by the fluorescence probe Nile Red. Membrane composition was altered by incorporation of cholesterol. Proton transport across membranes was estimated by the rate of decay of the pH gradient monitored by pyranine fluorescence. The fluorescence lifetime and lifetime distribution (analyzed by the maximum entropy method) of Nile Red in membranes were estimated. An increase in the level of cholesterol resulted ina decrease in the rate of proton transport and increases in both the peak value and width of the lifetime distribution of Nile Red. These results areinterpreted by a model wherein cholesterol causes a decrease in the water content of membranes and thereby decreases the probability of the H-bonded water wire across membranes, resulting in a decrease in proton flux.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 13:53:05