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Titolo:
Single-molecule study of an adsorbed oligonucleotide undergoing both lateral diffusion and strong adsorption
Autore:
Wirth, MJ; Swinton, DJ;
Indirizzi:
Univ Delaware, Dept Chem & Biochem, Newark, DE 19716 USA Univ Delaware Newark DE USA 19716 pt Chem & Biochem, Newark, DE 19716 USA
Titolo Testata:
JOURNAL OF PHYSICAL CHEMISTRY B
fascicolo: 7, volume: 105, anno: 2001,
pagine: 1472 - 1477
SICI:
1520-6106(20010222)105:7<1472:SSOAAO>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
FLUORESCENCE CORRELATION SPECTROSCOPY; PERFORMANCE LIQUID-CHROMATOGRAPHY; ACRIDINE-ORANGE; CONFORMATIONAL TRANSITIONS; WATER INTERFACES; RECOVERY; MICROSCOPY; MEMBRANES; MOBILITY; BEHAVIOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
36
Recensione:
Indirizzi per estratti:
Indirizzo: Wirth, MJ Univ Delaware, Dept Chem & Biochem, Newark, DE 19716 USA Univ Delaware Newark DE USA 19716 Biochem, Newark, DE 19716 USA
Citazione:
M.J. Wirth e D.J. Swinton, "Single-molecule study of an adsorbed oligonucleotide undergoing both lateral diffusion and strong adsorption", J PHYS CH B, 105(7), 2001, pp. 1472-1477

Abstract

Single-molecule fluorescence spectroscopy was used to probe the lateral transport of an adsorbed oligonucleotide, the SP6 promoter primer, which is a24-mer labeled at the 5' end with tetramethylrhodamine. The oligonucleotide was adsorbed to the interface of an aqueous solution of 0.01 M KC1 and silica chemically modified by chlorodimethyloctadecylsilane. Confocal fluorescence microscopy achieved single-molecule resolution, with a molecule in the beam 7% of the time. Autocorrelation of the data fit well to a model having two species, one diffusing and the other one undergoing transient strongadsorption. A small number of bursts, 0.3%, had unusually long durations, accounting for the slow component in the autocorrelation. When these long bursts were excised from the data, the autocorrelation fit well to simple diffusion, with D = 4 x 10(-6) cm(2)/s. Autocorrelation of the long bursts alone gave a rate constant for desorption of 3 s(-1). The strongly adsorbed molecules were found to comprise 10% of the total population of adsorbates. It is concluded that the lateral transport of the SP6 promoter primer is described by fast lateral diffusion interrupted by rare, reversible, strong adsorption to defect sites. During strong adsorption, the tetramethylrhodamine label undergoes hindered motion, suggesting it is not the adsorbing moiety.

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Documento generato il 01/04/20 alle ore 00:55:32