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Titolo:
Quantitative orientation of alpha-helical polypeptides by attenuated totalreflection infrared spectroscopy
Autore:
Buffeteau, T; Le Calvez, E; Desbat, B; Pelletier, I; Pezolet, M;
Indirizzi:
Univ Bordeaux 1, UMR 5803 CNRS, Lab Phys Chim Mol, F-33405 Talence, FranceUniv Bordeaux 1 Talence France F-33405 Chim Mol, F-33405 Talence, France Univ Laval, Dept Chim, Ctr Rech Sci & Ingn Macromol, Quebec City, PQ G1K 7P4, Canada Univ Laval Quebec City PQ Canada G1K 7P4 Quebec City, PQ G1K 7P4, Canada
Titolo Testata:
JOURNAL OF PHYSICAL CHEMISTRY B
fascicolo: 7, volume: 105, anno: 2001,
pagine: 1464 - 1471
SICI:
1520-6106(20010222)105:7<1464:QOOAPB>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
ANISOTROPIC OPTICAL-CONSTANTS; LANGMUIR-BLODGETT-FILMS; L-GLUTAMATE MONOLAYERS; FTIR-ATR SPECTROSCOPY; BENZYL-L-GLUTAMATE; TRANSMISSION SPECTROSCOPY; PHOSPHOLIPID MONOLAYERS; TRANSITION MOMENTS; MEMBRANE-PROTEINS; WATER-INTERFACE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Physical, Chemical & Earth Sciences
Citazioni:
56
Recensione:
Indirizzi per estratti:
Indirizzo: Buffeteau, T Univ Bordeaux 1, UMR 5803 CNRS, Lab Phys Chim Mol, F-33405 Talence, France Univ Bordeaux 1 Talence France F-33405 3405 Talence, France
Citazione:
T. Buffeteau et al., "Quantitative orientation of alpha-helical polypeptides by attenuated totalreflection infrared spectroscopy", J PHYS CH B, 105(7), 2001, pp. 1464-1471

Abstract

Spectral simulations of polarized attenuated total reflection (ATR) spectra have been used to quantitatively determine the orientation of alpha -helical polypeptides. Transmittance and polarized ATR spectra of five monolayers of poly-gamma -benzyl-L-glutamate (PBG) transferred by the Langmuir-Blodgett techniques on germanium crystal have been recorded, and the dichroic ratios of the amide I and amide II bands have been calculated. Simulations were performed using anisotropic optical constants of PEG in the molecular coordinate system and using the 4 x 4 matrix formalism of Berreman. Assuming that the alpha -helices are parallel to the plane of the ATR crystal, an average angle of 38 degrees +/- 1 degrees between the helix axes and the withdrawing direction has been found. Simulated spectra for various orientations of the alpha -helices are then given and influence of the azimuthal and tilt angles on the dichroic ratios of the amide bands has been investigated.

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Documento generato il 02/04/20 alle ore 10:30:15