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Titolo:
Quantitative imaging of tyrosine hydroxylase and calmodulin in the human brain
Autore:
Sutoo, D; Akiyama, K; Yabe, K;
Indirizzi:
Univ Tsukuba, Inst Med Sci, Tsukuba, Ibaraki 3058575, Japan Univ Tsukuba Tsukuba Ibaraki Japan 3058575 sukuba, Ibaraki 3058575, Japan
Titolo Testata:
JOURNAL OF NEUROSCIENCE RESEARCH
fascicolo: 5, volume: 63, anno: 2001,
pagine: 369 - 376
SICI:
0360-4012(20010301)63:5<369:QIOTHA>2.0.ZU;2-I
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNO-CYTOCHEMICAL LOCALIZATION; DEPENDENT PROTEIN-KINASE; MONOAMINE NEURON SYSTEMS; CENTRAL-NERVOUS-SYSTEM; RAT-BRAIN; IMMUNOHISTOCHEMICAL LOCALIZATION; TRYPTOPHAN-HYDROXYLASE; MICROPHOTOMETRY SYSTEM; SYNTHESIZING SYSTEM; PARKINSONS-DISEASE;
Keywords:
fluorescence microphotometry system; human brain mapping analyzer; quantitative immunohistochemistry;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Sutoo, D Univ Tsukuba, Inst Med Sci, Tsukuba, Ibaraki 3058575, Japan Univ Tsukuba Tsukuba Ibaraki Japan 3058575 baraki 3058575, Japan
Citazione:
D. Sutoo et al., "Quantitative imaging of tyrosine hydroxylase and calmodulin in the human brain", J NEUROSC R, 63(5), 2001, pp. 369-376

Abstract

The distributions of tyrosine hydroxylase and calmodulin in adult normal postmortem human brain were analyzed quantitatively. Consecutive coronal sections were obtained from the anterior area of the right hemisphere and werestained immunohistochemically for tyrosine hydroxylase and calmodulin. Stained sections were divided into approximately 3 million microareas at 50 mum intervals, and the immunohistochemical fluorescence intensity in each area was measured by a human brain mapping analyzer, which is a microphotometry system for analysis of the distribution of neurochemicals in a large tissue slice. Immunoreactive staining of tyrosine hydroxylase and calmodulin was observed in almost all brain regions, but its intensity varied. Relatively high levels of calmodulin were observed in brain regions with high levelsof tyrosine hydroxylase, though high levels of tyrosine hydroxylase were not always observed in brain regions where high levels of calmodulin were distributed. In particular, high levels of both of tyrosine hydroxylase and calmodulin were distributed in the caudate nucleus and putamen. Previously it was shown that tyrosine hydroxylase was activated and dopamine synthesis was enhanced in the neostriatum region in mice and rats by the intracerebroventricular administration of calcium through a calmodulin-dependent system. The present results combined with these previous findings suggest that the activity of tyrosine hydroxylase in the caudate nucleus and putamen of humans may also be regulated by a calcium/calmodulin-dependent system. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 02:24:33