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Titolo:
A ganglioside-specific sialyltransferase localizes to axons and non-Golgi structures in neurons
Autore:
Stern, CA; Tiemeyer, M;
Indirizzi:
Yale Univ, Sch Med, Dept Cell Biol, New Haven, CT 06510 USA Yale Univ NewHaven CT USA 06510 Dept Cell Biol, New Haven, CT 06510 USA
Titolo Testata:
JOURNAL OF NEUROSCIENCE
fascicolo: 5, volume: 21, anno: 2001,
pagine: 1434 - 1443
SICI:
0270-6474(20010301)21:5<1434:AGSLTA>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
CEREBELLAR GRANULE CELLS; NERVE GROWTH-FACTOR; INTEGRAL MEMBRANE-PROTEIN; RAT SYMPATHETIC NEURONS; DISULFIDE-BONDED DIMER; HIPPOCAMPAL-NEURONS; SIGNAL-TRANSDUCTION; TYROSINE PHOSPHORYLATION; SUBCELLULAR-LOCALIZATION; GA2/GM2/GD2 SYNTHASE;
Keywords:
ganglioside; neurons; PC12 cells; sialyltransferase; axon; glycolipid synthesis;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
101
Recensione:
Indirizzi per estratti:
Indirizzo: Tiemeyer, M Glyko Inc, 11 Pimentel Court, Novato, CA 94949 USA Glyko Inc 11 Pimentel Court Novato CA USA 94949 , CA 94949 USA
Citazione:
C.A. Stern e M. Tiemeyer, "A ganglioside-specific sialyltransferase localizes to axons and non-Golgi structures in neurons", J NEUROSC, 21(5), 2001, pp. 1434-1443

Abstract

To investigate the tissue distribution and subcellular localization of ST3GalV (CMP-NeuAc: lactosylceramide alpha2,3 sialyltransferase/GM3 synthase) in the adult mouse, we generated two antisera against mouse ST3GalV that were designated CS2 (directed against amino acids K227-I272) and CS14 (directed against amino acids D308-H359). We previously reported that CS2 antiserum stains medial and trans-Golgi cisternae in all cell types investigated. In neural tissue, however, CS14 antiserum reveals a subpopulation of ST3GalVwith a subcellular distribution complementary to CS2 antiserum. CS14 antiserum strongly stains axons in cortical, cerebellar, brainstem, and spinal cord tissue sections. The subcellular localization of neuronal ST3GalV is maintained in primary cultures of rat hippocampal neurons and in PC12 cells. In PC12 cells, ST3GalV localization evolves during NGF-induced differentiation such that a pool of enzyme leaves the Golgi for a distal compartment inconjunction with neurite outgrowth. In PC12 cells transfected with an epitope-tagged form of ST3GalV, staining for the epitope tag coincides with expression of endogenous enzyme. The non-Golgi pool of ST3GalV does not colocalize with markers for the trans-Golgi network, endosome, or synaptic vesicles, nor is it detected on the cell surface. Distinct subpopulations of ST3GalV imply that ganglioside synthesis can occur outside of the Golgi or, alternatively, that a portion of the total ST3GalV pool subserves a nonenzymatic function. Significantly fewer transfected cells were found in PC12 cultures treated with plasmid encoding ST3GalV than in cultures treated with control plasmid, indicating that the expression of ST3GalV in excess of endogenous levels results in either cell death or a decreased rate of cell division.

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Documento generato il 01/04/20 alle ore 02:10:31