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Titolo:
Evidence for direct involvement of beta-catenin in phorbol ester-induced neurite outgrowth in GT1-1 hypothalamic neurones
Autore:
Sun, W; Lee, H; Choe, Y; Cho, S; Kim, DH; Kim, K;
Indirizzi:
Seoul Natl Univ, Coll Nat Sci, Sch Biol Sci, Seoul 151742, South Korea Seoul Natl Univ Seoul South Korea 151742 Sci, Seoul 151742, South Korea Kwangju Inst Sci & Technol, Dept Life Sci, Kwangju, South Korea Kwangju Inst Sci & Technol Kwangju South Korea ci, Kwangju, South Korea
Titolo Testata:
JOURNAL OF NEUROENDOCRINOLOGY
fascicolo: 3, volume: 13, anno: 2001,
pagine: 249 - 260
SICI:
0953-8194(200103)13:3<249:EFDIOB>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
GONADOTROPIN-RELEASING-HORMONE; PROTEIN-KINASE-C; GAMMA-AMINOBUTYRIC-ACID; UBIQUITIN-PROTEASOME PATHWAY; CENTRAL-NERVOUS-SYSTEM; MESSENGER-RNA; N-CADHERIN; CELL-LINE; TYROSINE PHOSPHORYLATION; SEXUAL-MATURATION;
Keywords:
GnRH; gamma-aminobutyric acid; protein kinase C; beta-catenin; neurite outgrowth; differentiation;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
50
Recensione:
Indirizzi per estratti:
Indirizzo: Kim, K Seoul Natl Univ, Coll Nat Sci, Sch Biol Sci, Seoul 151742, South Korea Seoul Natl Univ Seoul South Korea 151742 eoul 151742, South Korea
Citazione:
W. Sun et al., "Evidence for direct involvement of beta-catenin in phorbol ester-induced neurite outgrowth in GT1-1 hypothalamic neurones", J NEUROENDO, 13(3), 2001, pp. 249-260

Abstract

Gonadotropin-releasing hormone (GnRH) is a pivotal neuroendocrine regulator controlling reproductive functions. However, the scattered distribution of GnRH neurones in the mammalian brain has hindered studies on the development and differentiation of GnRH neurones. In the present study, we used theimmortalized GnRH-producing GT1-1 cells to examine whether activation of protein kinase C (PKC) pathway with 12-O-tetradecanoyl-13-acetate (TPA) induces morphological and functional differentiation of GnRH neurones. TPA induced neurite outgrowth and inhibited proliferation of GT1-1 cells that were specifically antagonized by cotreatment of PKC inhibitor, calphostin C. Thefunctional significance of TPA-induced differentiation of GT1-1 cells was manifested in part by the changes in the effects of aminobutyric acid (GABA) on intracellular Ca2+ levels. in untreated GT1-1 cells, activation of GABA-A receptor with 10 muM muscimol increased intracellular Ca2+ levels, whereas such stimulatory effects disappeared in GT1-1 cells bearing neurites. Accordingly, muscimol could not stimulate GnRH release in TPA-treated GT1-1 cells. To elucidate the molecular mechanism underlying TPA-induced neurite outgrowth, we performed differential display reverse transcription-polymerase chain reaction. Among several genes that are affected by TPA treatment, we found a significant induction of beta -catenin mRNA expression. Along with the rapid induction of beta -catenin protein levels, we observed that beta -catenin was reallocated from cell-cell adhesion sites to the growth cones within 3 h of TPA treatment. Transient transfection studies with green fluorescent protein as a reporter gene demonstrated that beta -catenin overexpression alone can promote neurite outgrowth in GT1-1 cells. Moreover, TPAwas found to increase the transcription-activational roles of beta -catenin. Together, these data provide evidence that beta -catenin is involved in the TPA-induced functional differentiation of immortalized GnRH neurones.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 07:02:10