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Titolo:
Serological diagnosis of Epstein-Barr virus infection by novel ELISAs based on recombinant capsid antigens p23 and p18
Autore:
Farber, I; Hinderer, W; Rothe, M; Long, D; Sonneborn, HH; Wutzler, P;
Indirizzi:
Univ Jena Klinikum, Inst Antivirale Chemotherapie, D-99089 Erfurt, GermanyUniv Jena Klinikum Erfurt Germany D-99089 rapie, D-99089 Erfurt, Germany Biotest AG, Res & Dev, Dreieich, Germany Biotest AG Dreieich GermanyBiotest AG, Res & Dev, Dreieich, Germany
Titolo Testata:
JOURNAL OF MEDICAL VIROLOGY
fascicolo: 4, volume: 63, anno: 2001,
pagine: 271 - 276
SICI:
0146-6615(200104)63:4<271:SDOEVI>2.0.ZU;2-K
Fonte:
ISI
Lingua:
ENG
Soggetto:
IMMUNOGLOBULIN-M ANTIBODIES; ENZYME IMMUNOASSAYS; MONONUCLEOSIS; SERODIAGNOSIS; EXPRESSION; PROTEIN;
Keywords:
enzyme-linked immunosorbent assay; Epstein-Barr virus; infectious mononucleosis; recombinant protein; serological diagnosis; viral capsid antigens;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
15
Recensione:
Indirizzi per estratti:
Indirizzo: Farber, I Univ Jena Klinikum, Inst Antivirale Chemotherapie, Nordhauser Str 78, D-99089 Erfurt, Germany Univ Jena Klinikum Nordhauser Str 78 Erfurt Germany D-99089 any
Citazione:
I. Farber et al., "Serological diagnosis of Epstein-Barr virus infection by novel ELISAs based on recombinant capsid antigens p23 and p18", J MED VIROL, 63(4), 2001, pp. 271-276

Abstract

A new pair of Epstein-Barr virus ELISAs (Biotest Anti-EBV VCA IgG and VCA IgM ELISA) was evaluated for usefulness for routine diagnosis of acute EBV infections. The ELISAs are based on two viral capsid antigens (VCA), p23 (BLRF2, full-length) and p18 (BFRF3, carboxy-half), that are combined by autologous gene fusion. In total, 179 sera were tested in direct comparison with classical VCA immunofluorescence assays (IFA). With the help of clinical data and additional reference serology, i.e., heterophile antibodies, anti-EA IgG (IFA) and anti-EBNA-1 IgG (ELISA), the patients were divided into the following categories: seronegatives (46), acute primary infections (67), previous infections (39), suspected reactivations (20) and constellations with intermediate serological patterns (7). The VCA IgG and VCA IgM ELISAs showed overall agreement to IFA of 95.0% and 94.4%, respectively. The calculated analytical performance (sensitivity; specificity) of VCA IgG and VCA IgM was 94.0%; 97.8% and 97.1%; 96.5%, respectively. A certain delay in seroconversion of anti-p23-p18 IgG may account for a significant difference in sensitivity of the VCA IgG ELISA between primary (88.4%) and previous infections(100%). In summary, the new recombinant VCA ELISAs yielded good correlation to VCA IFA and in combination with EBNA-1 IgG allow rapid, sensitive,and specific diagnosis of infectious mononucleosis or EBV immune status ingeneral. (C) 2001 Wiley-Liss, Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 19:54:17