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Titolo:
Altered remodeling of nucleolar machineries in cultured hepatocytes treated with thioacetamide
Autore:
Jeong, JS; Han, SY; Kim, YH; Choi, YC;
Indirizzi:
Dong A Univ, Coll Med, Dept Pathol, Soe Gu, Busan 602103, South Korea DongA Univ Busan South Korea 602103 , Soe Gu, Busan 602103, South Korea Dong A Univ, Coll Med, Dept Internal Med, Busan 602103, South Korea Dong AUniv Busan South Korea 602103 rnal Med, Busan 602103, South Korea Dong A Univ, Coll Med, Dept Gen Surg, Busan 602103, South Korea Dong A Univ Busan South Korea 602103 Gen Surg, Busan 602103, South Korea Dong A Univ, Coll Med, Dept Pharmacol, Busan 602103, South Korea Dong A Univ Busan South Korea 602103 harmacol, Busan 602103, South Korea Dong A Univ, Coll Med, Inst Med Sci, Busan 602103, South Korea Dong A Univ Busan South Korea 602103 Med Sci, Busan 602103, South Korea
Titolo Testata:
JOURNAL OF KOREAN MEDICAL SCIENCE
fascicolo: 1, volume: 16, anno: 2001,
pagine: 75 - 82
SICI:
1011-8934(200102)16:1<75:ARONMI>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
MAMMALIAN HISTONE DEACETYLASE; RAT-LIVER; PROTEIN; GENE;
Keywords:
hepatocyte; thioacetamide; nucleolus organizer region; G2-arrest; regeneration;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
25
Recensione:
Indirizzi per estratti:
Indirizzo: Jeong, JS Dong A Univ, Coll Med, Dept Pathol, Soe Gu, 1-3 Dongdaesin Dong,Busan 602103, South Korea Dong A Univ 1-3 Dongdaesin Dong Busan South Korea 602103 Korea
Citazione:
J.S. Jeong et al., "Altered remodeling of nucleolar machineries in cultured hepatocytes treated with thioacetamide", J KOR MED S, 16(1), 2001, pp. 75-82

Abstract

Thioacetamide (TA) is converted into a hyperacetylating agent which causeshepatic necrosis, regeneration, cirrhosis and cancerous transformation. One of the most characteristic toxicities of TA in rat is observed with a 50 mg/kg per day which induces nucleolar enlargement different from that in regenerating liver. From TA treated liver, the nucleoli were isolated and characterized far an altered nucleolar signal transduction system. Immunochemistry revealed that the poisoned nucleoli had increased levels of both nucleolus specific proteins (nucleophosmin and nucleolin) and various signal molecules (CK2, Erk1/2, p38, protein kinases A and G, and cyclin A). Using flow cytometry, the nucleoli were found to be in G2-arrested nuclei. Manifestation of the nucleolar enlargement could be readily observed using an ex vivo hepatocyte culture. There were two types of nucleolar enlargement. One was observed in normal hepatocytes with light density of enlarged nucleoli. The other was in TA-treated hepatocytes with dense and compact density of enlarged nucleoli, which contained a 3 to 5-fold higher nucleophosmin contentthan the control. in vitro induction of nucleolar enlargement with TA was possible. As soon as the hepatocytes anchored on a collagen coat, exogeneous TA (higher than 1 mug/mL) could induce dense and compact nucleoli. However, when an exogeneous drug was added after monolayer formation (1 day), no drug-induced nucleolar enlargement was observed.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 05/04/20 alle ore 06:25:14