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Titolo:
Cell cycling status of human cord blood CD34+cells during ex vivo expansion is related to the level of very late antigen expression
Autore:
Seoh, JY; Park, HY; Chung, WS; Kim, SC; Hahn, MJ; Kim, KH; Shin, HY; Ahn, HS; Park, KW; Ryu, KH;
Indirizzi:
Ewha Womans Univ, Coll Med, Med Res Ctr, Dept Microbiol, Seoul 110120, South Korea Ewha Womans Univ Seoul South Korea 110120 iol, Seoul 110120, South Korea Ewha Womans Univ, Coll Med, Med Res Ctr, Dept Biochem, Seoul 110120, SouthKorea Ewha Womans Univ Seoul South Korea 110120 chem, Seoul 110120, SouthKorea Ewha Womans Univ, Coll Med, Med Res Ctr, Dept Clin Pathol, Seoul 110120, South Korea Ewha Womans Univ Seoul South Korea 110120 hol, Seoul 110120, South Korea Ewha Womans Univ, Coll Med, Med Res Ctr, Dept Obstet & Gynecol, Seoul 110120, South Korea Ewha Womans Univ Seoul South Korea 110120 col, Seoul 110120, South Korea Ewha Womans Univ, Coll Med, Med Res Ctr, Dept Pediat, Seoul 110120, South Korea Ewha Womans Univ Seoul South Korea 110120 iat, Seoul 110120, South Korea Sung Kyun Kwan Univ, Coll Med, Dept Microbiol, Suwon 440746, South Korea Sung Kyun Kwan Univ Suwon South Korea 440746 , Suwon 440746, South Korea Seoul Natl Univ, Coll Med, Dept Pedistr, Seoul, South Korea Seoul Natl Univ Seoul South Korea Med, Dept Pedistr, Seoul, South Korea Seoul Natl Univ, Coll Med, Dept Pediat Surg, Seoul, South Korea Seoul NatlUniv Seoul South Korea Dept Pediat Surg, Seoul, South Korea
Titolo Testata:
JOURNAL OF KOREAN MEDICAL SCIENCE
fascicolo: 1, volume: 16, anno: 2001,
pagine: 20 - 24
SICI:
1011-8934(200102)16:1<20:CCSOHC>2.0.ZU;2-R
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADHESION MOLECULES; PERIPHERAL-BLOOD; BONE-MARROW; IN-VITRO; INTEGRIN; MOBILIZATION; PRIMATES;
Keywords:
VLA-4; receptors, very late antigen; cell cycle; fetal blood; antigens, CD34; ex vivo expansion;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
13
Recensione:
Indirizzi per estratti:
Indirizzo: Ryu, KH Ewha Womans Univ, Dongdaemun Hosp, Coll Med, Dept Pediat,Chongro Gu, 70 Chongro 6 Ga, Seoul 110120, South Korea Ewha Womans Univ 70 Chongro 6Ga Seoul South Korea 110120 h Korea
Citazione:
J.Y. Seoh et al., "Cell cycling status of human cord blood CD34+cells during ex vivo expansion is related to the level of very late antigen expression", J KOR MED S, 16(1), 2001, pp. 20-24

Abstract

Very late antigen-4 (VLA-4), which binds to the extracellular matrix protein fibronectin, is an integrin molecule known to be modulated during mobilization of CD34+ cells, and to be involved in signaling the mobilization stimuli, On the hypothesis that cell cycling status might be different depending on the level of VLA-4 expression, we investigated the DNA contents of human cord blood CD34+ cells during ex vivo expansion by recombinant human thrombopoietin and flt3-ligand with simultaneous measurement of surface VLA-4at the 1st and 4th week. During this ex vivo expansion, expression of VLA-4 increased and almost all cells became VLA-4+ until the 4th day of culture, Expression of VLA-4 was maintained in the major population of the cultured cells until the 4th week, The cells in S/G2/M phase were greater in number in VLA-4 high fraction than in VLA-4 low fraction (n=4, p<.001). Furthermore, the fraction of cells in S/G2/M phase increased as the expression of VLA-4 became higher, These results suggest that cord blood CD34+ cells expressing high levels of VLA-4 have more proliferative activities.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 08:27:16