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Titolo:
Increased sensitivity to glucocorticoids in peripheral blood mononuclear cells of chronic fatigue syndrome patients, without evidence for altered density or affinity of glucocorticoid receptors
Autore:
Visser, J; Lentjes, E; Haspels, I; Graffelman, W; Blauw, B; de Kloet, R; Nagelkerken, L;
Indirizzi:
TNO, Div Immunol & Infect Dis, NL-2301 CE Leiden, Netherlands TNO LeidenNetherlands NL-2301 CE ct Dis, NL-2301 CE Leiden, Netherlands Leiden Amsterdam Ctr Drug Res, Div Med Pharmacol, Leiden, Netherlands Leiden Amsterdam Ctr Drug Res Leiden Netherlands l, Leiden, Netherlands Leiden Univ, Ctr Med, Dept Clin Chem, Leiden, Netherlands Leiden Univ Leiden Netherlands Med, Dept Clin Chem, Leiden, Netherlands Leiden Univ, Ctr Med, Dept Gen Practice & Nursing Home Med, Leiden, Netherlands Leiden Univ Leiden Netherlands & Nursing Home Med, Leiden, Netherlands
Titolo Testata:
JOURNAL OF INVESTIGATIVE MEDICINE
fascicolo: 2, volume: 49, anno: 2001,
pagine: 195 - 204
SICI:
1081-5589(200103)49:2<195:ISTGIP>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
PITUITARY-ADRENAL AXIS; CORTICOTROPIN-RELEASING FACTOR; POSTTRAUMATIC-STRESS-DISORDER; FACTOR-LIKE IMMUNOREACTIVITY; LOW-BACK-PAIN; TRANSCRIPTIONAL ACTIVATION; DEPRESSED-PATIENTS; T-CELLS; DEXAMETHASONE; FIBROMYALGIA;
Keywords:
glucocorticoids; glucocorticoid receptors; chronic fatigue syndrome; cytokines;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Nagelkerken, L TNO, Div Immunol & Infect Dis, POB 2215, NL-2301 CE Leiden,Netherlands TNO POB 2215 Leiden Netherlands NL-2301 CE en, Netherlands
Citazione:
J. Visser et al., "Increased sensitivity to glucocorticoids in peripheral blood mononuclear cells of chronic fatigue syndrome patients, without evidence for altered density or affinity of glucocorticoid receptors", J INVES MED, 49(2), 2001, pp. 195-204

Abstract

Background: In this study we tested the hypothesis that the increased sensitivity to glucocorticoids in chronic fatigue syndrome (CFS)-patients can be attributed to an altered functioning of their glucocorticoid receptors (GR),Methods: For this purpose, affinity and distribution of the GR were studied in purified, peripheral blood mononuclear cells (PBMC) of 10 CFS patientsand 14 controls along with the responsiveness of these cells to glucocorticoids in vitro,Results: Affinity (Kd) and number of GR was not different in PBMC of CFS patients when compared with the controls (Kd, 12.9+/-8.9 nmol vs 18.8+/-16.2nmol and GR number, 4839+/-2824/ cell vs 4906+/-1616/cell), Moreover, RT-PCR revealed no differences in GR messenger RNA expression, Nevertheless, PBMC from CFS patients showed an increased sensitivity to glucocorticoids in vitro. In CFS patients 0.01 mu mol dexamethasone suppressed PBMC proliferation by 37%, whereas the controls were only suppressed by 17% (P<0.01). Addition of phorbol 12-myristate 13-acetate to the cultures rendered the cells resistant to dexamethasone with regard to proliferation and IL-10 and IFN-<gamma> production, but not to IL-2 and TNF-alpha production in both patients and controls, No difference between patients and controls,vas observed inthis respect. Conclusions: In conclusion, PBMC of CFS patients display an increased sensitivity to glucocorticoids, which cannot be explained by number or affinityof the GR but should rather be attributed to molecular processes beyond the actual binding of the ligand to the GR.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/10/20 alle ore 14:43:09