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Titolo:
Interleukin-1 beta gene transcription in U937 cells is modulated by type Icollagen and cytoskeletal integrity via distinct signaling pathways
Autore:
Ritzenthaler, JD; Roman, J;
Indirizzi:
Atlanta Vet Affairs Med Ctr, Dept Med, Div Pulm & Crit Care, Atlanta, GA 30033 USA Atlanta Vet Affairs Med Ctr Atlanta GA USA 30033 e, Atlanta, GA 30033 USA Emory Univ, Sch Med, Atlanta, GA 30033 USA Emory Univ Atlanta GA USA 30033 mory Univ, Sch Med, Atlanta, GA 30033 USA
Titolo Testata:
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
fascicolo: 2, volume: 21, anno: 2001,
pagine: 105 - 116
SICI:
1079-9907(200102)21:2<105:IBGTIU>2.0.ZU;2-W
Fonte:
ISI
Lingua:
ENG
Soggetto:
BLOOD MONONUCLEAR-CELLS; NECROSIS-FACTOR-ALPHA; PROTEIN-KINASE-C; PLASMINOGEN-ACTIVATOR GENE; MESSENGER-RNA EXPRESSION; EXTRACELLULAR-MATRIX; ACTIN CYTOSKELETON; FOCAL ADHESIONS; MONOCYTIC CELLS; RECEPTOR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
55
Recensione:
Indirizzi per estratti:
Indirizzo: Roman, J Atlanta Vet Affairs Med Ctr, Dept Med, Pulm & Crit Care Sect, 111,Room 12C191,1670 Clairmont Rd, Decatur, GA 30033 USA Atlanta Vet Affairs Med Ctr 111,Room 12C 191,1670 Clairmont Rd Decatur GA USA 30033
Citazione:
J.D. Ritzenthaler e J. Roman, "Interleukin-1 beta gene transcription in U937 cells is modulated by type Icollagen and cytoskeletal integrity via distinct signaling pathways", J INTERF CY, 21(2), 2001, pp. 105-116

Abstract

Type I collagen (Col), an extracellular matrix molecule highly expressed in injured tissues, stimulates interleukin-1 beta (IL-1 beta) expression in monocytic cells. Using U937 cells transfected with the human IL-1 beta genepromoter connected to a reporter gene, we examined how the organizational state of the cytoskeleton modulates the expression of IL-1 beta after Col stimulation. We found the same degree of stimulation of IL-1 beta gene transcription in cells exposed to Col presented in different fashions (i.e., soluble Col, Col-coated plate, three-dimensional Col lattice), suggesting thatstimulation of IL-1 beta is independent of the mode of presentation of Col, The Col-stimulated response was associated with induction of the transcription factor activator protein-1 (AP1) and was abolished by a protein kinase C (PKC) inhibitor, by a mitogen-activated protein kinase (MAPK) inhibitor, and by cotransfection of cells with a competing AP-1 oligo, Disruption ofcytoskeletal organization with colchicine or cytochalasin B stimulated IL-1 beta gene transcription and enhanced the cells' response to Col, The effects of cytochalasin and cochicine were inhibited by the PKC inhibitor but were not affected by the MAPK inhibitor or the AP-1 oligo, These findings suggest that the cytoskeletal integrity modulates the constitutive and Col-stimulated transcription of the IL-1 beta gene via distinct signaling mechanisms.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 15:34:12