Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Modulation of functional activities of chicken heterophils by recombinant chicken IFN-gamma
Autore:
Kogut, MH; Pishko, EJ; Kaspers, B; Weining, KC;
Indirizzi:
USDA ARS, Food Anim Protect Res Lab, So Plains Agr Res Ctr, College Stn, TX 77845 USA USDA ARS College Stn TX USA 77845 Agr Res Ctr, College Stn, TX 77845 USA Univ Munich, Inst Tierphysiol, Munich, Germany Univ Munich Munich Germany iv Munich, Inst Tierphysiol, Munich, Germany Univ Freiburg, Inst Med Mikrobiol & Hyg, Abt Virol, Freiburg, Germany UnivFreiburg Freiburg Germany biol & Hyg, Abt Virol, Freiburg, Germany
Titolo Testata:
JOURNAL OF INTERFERON AND CYTOKINE RESEARCH
fascicolo: 2, volume: 21, anno: 2001,
pagine: 85 - 92
SICI:
1079-9907(200102)21:2<85:MOFAOC>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
ENTERITIDIS-IMMUNE LYMPHOKINES; BOVINE INTERFERON-GAMMA; POLYMORPHONUCLEAR LEUKOCYTES; BACTERICIDAL ACTIVITIES; NEUTROPHIL FUNCTION; CHEMOTACTIC FACTORS; OXIDATIVE BURST; ACTIVATION; RESPONSES; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Kogut, MH USDA ARS, Food Anim Protect Res Lab, So Plains Agr Res Ctr, 2881F&B Rd, College Stn, TX 77845 USA USDA ARS 2881 F&B Rd College Stn TX USA 77845 Stn, TX 77845 USA
Citazione:
M.H. Kogut et al., "Modulation of functional activities of chicken heterophils by recombinant chicken IFN-gamma", J INTERF CY, 21(2), 2001, pp. 85-92

Abstract

The objective of the present studies was to examine the in vitro effects of recombinant chicken interferon-gamma (rChIFN-gamma) on shape change, phagocytosis, and the oxidative/nonoxidative killing activities of day-old chicken heterophils, Heterophils (4 x 10(6)/ml) were incubated with various concentrations of recombinant ChIFN-gamma from both Escherichia coil and transfected Cos cells for 2 h at 39 degreesC, The incubation of the neonatal heterophils with rChIFN-gamma resulted in significantly greater numbers of cells with membrane shape change when compared with the mock-treated heterophils, Both Cos cell-derived and E, coli-derived ChIFN-gamma significantly increased (p < 0.01) the phagocytosis of opsonized or nonopsonized Salmonella enteritidis by the neonatal heterophils in a concentration-dependent manner. Incubation with ChIFN-<gamma> induced no direct stimulation of the respiratory burst by the chicken heterophils but did prime the heterophils for a significantly strengthened respiratory burst to subsequent stimulation withopsonized zymosan (OZ), Lastly, incubation of the heterophils with ChIFN-gamma primed the cells for a significant increase in the release of beta -D-glucuronidase following stimulation with OZ. These results show that neonatal avian heterophils can respond to cytokine modulation with enhanced functional competence, suggesting that ChIFN-gamma can enhance the immune competence of the innate defenses of chickens during the first week of life.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 15/01/21 alle ore 23:19:00