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Titolo:
A rapid, novel strategy to induce tumor cell-specific cytotoxic T lymphocyte responses using instant dendritomas
Autore:
Holmes, LM; Li, JH; Sticca, RP; Wagner, TE; Wei, YZ;
Indirizzi:
Greenville Hosp Syst, Oncol Res Inst, Greenville, SC 29605 USA Greenville Hosp Syst Greenville SC USA 29605 st, Greenville, SC 29605 USA Clemson Univ, Dept Microbiol & Mol Med, Clemson, SC USA Clemson Univ Clemson SC USA v, Dept Microbiol & Mol Med, Clemson, SC USA
Titolo Testata:
JOURNAL OF IMMUNOTHERAPY
fascicolo: 2, volume: 24, anno: 2001,
pagine: 122 - 129
SICI:
1524-9557(200103/04)24:2<122:ARNSTI>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
THERAPEUTIC ANTITUMOR IMMUNITY; IN-VIVO; ANTIGEN; MODEL; VACCINATION; GENERATION; CARCINOMA; MELANOMA; VITRO;
Keywords:
dendritic cells; fusion; melanoma; immunotherapy;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
26
Recensione:
Indirizzi per estratti:
Indirizzo: Wei, YZ Greenville Hosp Syst, Oncol Res Inst, 900 W Faris Rd, Greenville, SC 29605USA Greenville Hosp Syst 900 W Faris Rd Greenville SC USA 29605 605USA
Citazione:
L.M. Holmes et al., "A rapid, novel strategy to induce tumor cell-specific cytotoxic T lymphocyte responses using instant dendritomas", J IMMUNOTH, 24(2), 2001, pp. 122-129

Abstract

The generation of fused cells between dendritic cells (DC) and tumor cellsis a very effective approach for tumor antigen presentation in cancer immunotherapy. However, the application of this approach in clinical studies islimited by the need for established tumor cell lines and the time-consuming procedures fur selecting and expanding the fused cells. In the current study, the authors report a rapid, novel approach to produce fused cells between DCs and primary tumor cells from patients with malignant melanoma. Peripheral blood DCs and a primary tumor cell culture were generated from the same patients, labeled with fluorescent green and red dyes, respectively, and fused. The fused cells were isolated by fluorescence-activated cell sorting. Because the fused cells do not need to be expanded, these cell hybrids have been named instant dendritomas. Fluorescence-activated cell sorting analysis showed that instant dendritomas express the key molecules for antigen presentation (HLA-A, B, C: HLA-DR; CD80; and CD86). In vitro studies haveshown that instant dendritomas effectively activated autologous CD8(+) T lymphocytes to proliferate and secret interferon-gamma. More importantly, the activated CD8(+) T lymphocytes effectively lysed the patients' primary tumor cells. This approach represents a practical clinical strategy for cancer immunotherapy.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 02/04/20 alle ore 00:05:03