Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Identification of DMC1, a novel gene in the TOC region on 17q25.1 that shows loss of expression in multiple human cancers
Autore:
Harada, H; Nagai, NH; Tsuneizumi, M; Mikami, I; Sugano, S; Emi, M;
Indirizzi:
Nippon Med Sch, Inst Gerontol, Dept Mol Biol, Nakahara Ku, Kawasaki, Kanagawa 2118533, Japan Nippon Med Sch Kawasaki Kanagawa Japan 2118533 i, Kanagawa 2118533, Japan Univ Tokyo, Inst Med Sci, Dept Virol, Tokyo, Japan Univ Tokyo Tokyo Japan iv Tokyo, Inst Med Sci, Dept Virol, Tokyo, Japan
Titolo Testata:
JOURNAL OF HUMAN GENETICS
fascicolo: 2, volume: 46, anno: 2001,
pagine: 90 - 95
SICI:
1434-5161(2001)46:2<90:IODANG>2.0.ZU;2-J
Fonte:
ISI
Lingua:
ENG
Soggetto:
SPORADIC BREAST-TUMORS; EUKARYOTIC MESSENGER-RNAS; ALLELIC LOSS; RECEPTOR; LOCUS; POLYMORPHISM; CARCINOMAS; CLONING; CDNA; LOCALIZATION;
Keywords:
tumor suppressor gene; loss of heterozygosity; chromosome 17q; DMC1; TOC; familial focal nonepidermolytic palmoplantar keratoderma;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Emi, M Nippon Med Sch, Inst Gerontol, Dept Mol Biol, Nakahara Ku, 1-396 Kosugi Cho, Kawasaki, Kanagawa 2118533, Japan Nippon Med Sch 1-396 Kosugi ChoKawasaki Kanagawa Japan 2118533 pan
Citazione:
H. Harada et al., "Identification of DMC1, a novel gene in the TOC region on 17q25.1 that shows loss of expression in multiple human cancers", J HUM GENET, 46(2), 2001, pp. 90-95

Abstract

Frequent allelic losses within chromosomal band 17q25.1 in a variety of human cancers have suggested the presence of one or more tumor suppressor genes in this region. Furthermore, a genetic locus responsible for familial focal nonepidermolytic palmoplantar keratoderma, a condition associated with cancer of the esophagus, lies in the same region. This esophageal-cancer susceptibility locus, TOC (tylosis with oesophageal cancer), might be a target of deletions at 17q25.1 in multiple types of malignancy. Using the reverse transcriptase-polymerase chain reaction (RT PCR) to examine cancer cell lines for alterations in the expression of transcripts from this portion of 17q, we identified a novel gene that we designated DMC1 (downregulated in multiple cancer-1). The full-length cDNA is 3293 bp long. its putative product is an integral membrane protein of 788 amino acids, belonging to the class of so-called "inside-out" membrane proteins; it lacks a signal sequence but contains an N-terminal cytoplasmic domain, a single transmembrane peptide, and a C-terminal extracellular domain. We documented loss of expressionof DMC1 in 2 of 10 breast-cancer cell lines, in 7 of 10 cervical-cancer lines, in 7 of 13 hepatocellular-cancer lines, in 3 of 7 lung-cancer lines, in 3 of 6 thyroid-cancer lines, in 2 of 6 gastric-cancer lines, and in 2 of 4 renal cell-cancer lines. Our results suggest that loss of expression of the DMC1 gene at 17q25.1 may pray an important role in the development of cancers in a broad range of human tissues.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/06/20 alle ore 01:17:09