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Titolo:
Gonadotropin-induced steroidogenic shift towards maturation-inducing hormone in Japanese yellowtail during final oocyte maturation
Autore:
Rahman, MA; Ohta, K; Chuda, H; Nakano, S; Maruyama, K; Matsuyama, M;
Indirizzi:
Kyushu Univ, Grad Sch Bioresource & Bioenvironm Sci, Fukuoka 8128581, Japan Kyushu Univ Fukuoka Japan 8128581 ioenvironm Sci, Fukuoka 8128581, Japan Nagasaki Prefectural Inst Fisheries, Nagasaki 8512213, Japan Nagasaki Prefectural Inst Fisheries Nagasaki Japan 8512213 512213, Japan Japan Sea Farming Assoc, Goto Stn, Nagasaki 8530501, Japan Japan Sea Farming Assoc Nagasaki Japan 8530501 , Nagasaki 8530501, Japan
Titolo Testata:
JOURNAL OF FISH BIOLOGY
fascicolo: 2, volume: 58, anno: 2001,
pagine: 462 - 474
SICI:
0022-1112(200102)58:2<462:GSSTMH>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
ATLANTIC CROAKER OOCYTES; MEDAKA ORYZIAS-LATIPES; INVITRO PRODUCTION; RAINBOW-TROUT; PLEURONECTES-PLATESSA; ONCORHYNCHUS-MYKISS; OVARIAN FOLLICLES; 17-ALPHA,20-BETA,21-TRIHYDROXY-4-PREGNEN-3-ONE; 17-ALPHA,20-BETA-DIHYDROXY-4-PREGNEN-3-ONE; OVULATION;
Keywords:
17,20 beta-dihydroxy-4-pregnen-3-one; oestradiol-17 beta; steroidogenic changes; HCG; teleost;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Matsuyama, M Kyushu Univ, Grad Sch Bioresource & Bioenvironm Sci, Fukuoka 8128581, Japan Kyushu Univ Fukuoka Japan 8128581 i, Fukuoka 8128581, Japan
Citazione:
M.A. Rahman et al., "Gonadotropin-induced steroidogenic shift towards maturation-inducing hormone in Japanese yellowtail during final oocyte maturation", J FISH BIOL, 58(2), 2001, pp. 462-474

Abstract

Intact ovarian follicles, obtained from untreated and human chorionic gonadotropin (HCG) treated Japanese yellowtail Seriola quinyueradiata during different maturational stages, were incubated With radioactive [H-3]pregnenolone, [H-3]17-hydroxypropesterone or [C-14] androstenedione and steroid metabolites identified by thin layer chromatography (TLC) followed by recrystallization to constant specific activity. In untreated late vitellogenic (0 h) follicles. androstenedione was the major product with smaller amounts of testosterone and oestradiol-17 beta. In post-vitellogenic (12h post-injection) intact follicles, androstenedione pre dominated, and although testosterone and oestradiol-17 beta were not produced, there were small amounts of 17.20 beta -dihydroxy-4-pregnen-3-one (17,20 beta -P) and 17,21-dihydroxy-4-pregnene 3,20-dione (11-deoxycortisol). In HCG-treated fish, a steroidogenic shift resulted in the disappearance of testosterone and oestradiol-17 beta coinciding with the appearance of 17,20 beta -P. During early and late final oocyte maturation FOM (24 and 36 h post-injection), there was a five- to seven-fold increase in the production of 17,20 beta -P, whereas production of 11-deoxycortisol remained almost the same. During FOM, in addition to 17,20 beta -P, its 5 beta -reduced metabolite, 17,20 beta -dihydroxy-5 beta-pregnan-3-one (5 beta -17,20 beta -P) was synthesized, suggesting a decrease in maturation-inducing 17,20 beta -P activity. 17,20 beta ,21 -Trihydroxy-4-pregnen-3-one (20 beta -S) was not synthesized by ovarian fragments inJapanese yellowtail at any maturational stage. The metabolites identified on TLC during FOM were tested to evaluate their maturation-inducing activity in an in vitro bioassay. Of the steroids tested, 17,20 beta -P was the most effective inducer of germinal vesicle breakdown (GVBD), followed by 5 beta -17,20 beta -P. Timely synthesis of 17,20 beta -P immediately prior to and during FOM as well as its great potency in inducing GVBD in vitro supports the evidence for a physiological role of 17,20 beta -P as a maturation-inducing hormone in Japanese yellowtail. (C) 2001 The Fisheries Society of the British Isles.

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Documento generato il 29/09/20 alle ore 23:46:35