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Titolo:
The expression of the IGF family and GH receptor in the bovine mammary gland
Autore:
Plath-Gabler, A; Gabler, C; Sinowatz, F; Berisha, B; Schams, D;
Indirizzi:
Tech Univ Munich, Inst Physiol, D-85350 Freising, Germany Tech Univ Munich Freising Germany D-85350 iol, D-85350 Freising, Germany Penn State Univ, Dept Dairy & Anim Sci, John O Almquist Res Ctr, University Pk, PA 16802 USA Penn State Univ University Pk PA USA 16802 r, University Pk, PA 16802 USA Univ Munich, Inst Anim Anat 2, D-80539 Munich, Germany Univ Munich Munich Germany D-80539 Anim Anat 2, D-80539 Munich, Germany
Titolo Testata:
JOURNAL OF ENDOCRINOLOGY
fascicolo: 1, volume: 168, anno: 2001,
pagine: 39 - 48
SICI:
0022-0795(200101)168:1<39:TEOTIF>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-FACTOR-I; FACTOR BINDING-PROTEINS; MESSENGER-RNA; LACTATION; PREGNANCY; INVITRO; TISSUE; RAT; SOMATOTROPIN; AUTOCRINE;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
41
Recensione:
Indirizzi per estratti:
Indirizzo: Schams, D Tech Univ Munich, Inst Physiol, Weihenstephaner Berg 3, D-85350 Freising, Germany Tech Univ Munich Weihenstephaner Berg 3 Freising GermanyD-85350
Citazione:
A. Plath-Gabler et al., "The expression of the IGF family and GH receptor in the bovine mammary gland", J ENDOCR, 168(1), 2001, pp. 39-48

Abstract

To study the involvement of the IGFs in mammary development and lactation of the cow, the temporal expressions of IGF-I and -II, its receptor type 1 (IGFR-1), IGF-binding proteins (IGFBPs)-1 to -6 and GH receptor (GHR) mRNA were examined. This was carried out for different stages of mammogenesis, lactogenesis, galactopoiesis and involution in the bovine mammary gland of 26 animals. Furthermore, IGF-I was localised by immunohistochemistry. The highest mRNA concentrations for IGF-I were detected ill the mammary tissue oflate pregnant heifers (days 255-272) and significantly lower expression was detected during lactogenesis and galactopoiesis. Immunohistochemistry of IGF-I revealed only a weak staining in the epithelium of the ducts during mammogenesis. The epithelium of the alveoli were negative during mammogenesis, lactogenesis and galactopoiesis but displayed distinct IGF-I activity during involution. In the stroma a distinct staining of the cytoplasm of adipocytes and of vascular smooth muscle cells was observed. A certain percentage of fibroblasts (usually 20-30%) were also immunopositive. In contrast, highest expression for IGFR-1 was detected during galactopoiesis and involution. The lowest mRNA concentration for IGFR-1 was found during pregnancy (days 194-213). In general, the expression of IGF-II was not regulated duringmammogenesis and lactation, but decreased during involution. The mRNA for the six binding proteins was detected in the bovine mammary gland. The dominant binding proteins were IGFBP-3 and -5. The highest expression of IGFBP-3 was observed during mid-pregnancy and the lowest during late lactation, involution and in nonpregnant heifers. The mRNA for IGFBP-5 increased duringlate mammogenesis and lactogenesis followed by a decrease thereafter. In general, the mRNA concentrations for IGFBP-2, -4 and -6 were barely detectable during all stages. In contrast, the expression for IGFBP-1 was upregulated in the mammary gland of virgin heifers and increased around the onset oflactation. mRNA for GHR was found during all stages examined without outstanding fluctuations. In conclusion, locally produced IGF-I and -II may mediate mammogenesis. The high mammary IGFR-1 mRNA during lactation suggests a role for peripheral IGF-I in maintenance of lactation. The role of IGFBPs in the mammary gland needs further evaluation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/12/20 alle ore 15:59:25