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Titolo:
Differential expression of the noradrenaline transporter in adrenergic chromaffin cells, ganglion cells and nerve fibres of the rat adrenal medulla
Autore:
Phillips, JK; Dubey, R; Sesiashvilvi, E; Takeda, M; Christie, DL; Lipski, J;
Indirizzi:
Univ Auckland, Sch Med, Dept Physiol, Auckland, New Zealand Univ AucklandAuckland New Zealand Dept Physiol, Auckland, New Zealand Univ Auckland, Sch Biomed Sci, Auckland, New Zealand Univ Auckland Auckland New Zealand ch Biomed Sci, Auckland, New Zealand
Titolo Testata:
JOURNAL OF CHEMICAL NEUROANATOMY
fascicolo: 1, volume: 21, anno: 2001,
pagine: 95 - 104
SICI:
0891-0618(200101)21:1<95:DEOTNT>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
ROSTRAL VENTROLATERAL MEDULLA; DOPAMINE-BETA-HYDROXYLASE; MESSENGER-RNA; NOREPINEPHRINE TRANSPORTER; SEROTONIN TRANSPORTER; TYROSINE-HYDROXYLASE; MOLECULAR-CLONING; PC12 CELLS; CATECHOLAMINE TRANSPORTERS; POSTNATAL-DEVELOPMENT;
Keywords:
catecholamines; type I ganglion cells; GAP-43; tyrosine hydroxylase; phenylethanolamine N-methyltransferase; immunohistochemistry; reverse transcription-polymerase chain reaction;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
59
Recensione:
Indirizzi per estratti:
Indirizzo: Phillips, JK Univ Auckland, Sch Med, Dept Physiol, Private Bag 92019, Auckland, New Zealand Univ Auckland Private Bag 92019 Auckland New Zealand ealand
Citazione:
J.K. Phillips et al., "Differential expression of the noradrenaline transporter in adrenergic chromaffin cells, ganglion cells and nerve fibres of the rat adrenal medulla", J CHEM NEUR, 21(1), 2001, pp. 95-104

Abstract

Expression of the noradrenaline transporter (NAT) was identified in various cell and fibre populations of the rat adrenal medulla, examined with immunohistochemistry and confocal microscopy. Immunoreactivity for the catecholamine biosynthetic enzymes tyrosine hydroxylase (TH), aromatic-L-amino-aciddecarboxylase (AADC) and dopamine beta -hydroxylase (DBH) was present in all chromaffin cells, while phenylethanolamine N-methyltransferase (PNMT) was used to determine adrenergic chromaffin cell groups. Labelling with NAT antibody was predominantly cytoplasmic and colocalised with PNMT immunoreactivity. Noradrenergic chromaffin cells were not NAT immunoreactive. Additionally, NAT antibody labelling demonstrated clusters of ganglion cells (presumably Type I) and nerve fibres. Expression of TH, AADC, DBH, PNMT and NAT mRNA was examined using reverse transcription-polymerase chain reaction (RT-PCR) from adrenal medulla punches and single chromaffin cells, and results were consistent with those obtained with immunocytochemistry. Chromaffin cells and fibres labelled with antibodies against growth associated protein-43 (GAP-43) were not NAT immunoreactive, while ganglion cells were doubled labelled with the two antibodies. The presence of NAT in adrenergic chromaffin cells, and its absence from noradrenergic cells, suggests that the adrenergic cell type is primarily responsible for uptake of catecholamines in the adrenal medulla. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/01/20 alle ore 21:08:10