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Titolo:
Rab27a regulates the peripheral distribution of melanosomes in melanocytes
Autore:
Hume, AN; Collinson, LM; Rapak, A; Gomes, AQ; Hopkins, CR; Seabra, MC;
Indirizzi:
Univ London Imperial Coll Sci Technol & Med, Sch Med, Div Biomed Sci, Cell& Mol Biol Sect, London SW7 2AZ, England Univ London Imperial Coll Sci Technol & Med London England SW7 2AZ gland Univ London Imperial Coll Sci Technol & Med, Sch Med, Dept Biochem, LondonSW7 2AZ, England Univ London Imperial Coll Sci Technol & Med London England SW7 2AZ gland
Titolo Testata:
JOURNAL OF CELL BIOLOGY
fascicolo: 4, volume: 152, anno: 2001,
pagine: 795 - 808
SICI:
0021-9525(20010219)152:4<795:RRTPDO>2.0.ZU;2-A
Fonte:
ISI
Lingua:
ENG
Soggetto:
HERMANSKY-PUDLAK-SYNDROME; MYOSIN-V; GERANYLGERANYL TRANSFERASE; SUBCELLULAR-LOCALIZATION; MOUSE MELANOCYTES; VESICLE TRANSPORT; COAT-COLOR; MEMBRANE-TRANSPORT; SMALL GTPASES; WILD-TYPE;
Keywords:
Rab27a; GTP-binding proteins; vesicular transport; melanosome; myosinVa;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
58
Recensione:
Indirizzi per estratti:
Indirizzo: Seabra, MC Univ London Imperial Coll Sci Technol & Med, Sch Med, Div Biomed Sci, Cell& Mol Biol Sect, Sir Alexander Fleming Bldg, London SW7 2AZ, England Univ London Imperial Coll Sci Technol & Med Sir Alexander Fleming BldgLondon England SW7 2AZ
Citazione:
A.N. Hume et al., "Rab27a regulates the peripheral distribution of melanosomes in melanocytes", J CELL BIOL, 152(4), 2001, pp. 795-808

Abstract

Rab GTPases are regulators of intracellular membrane traffic. We report a possible function of Rab27a, a protein implicated in several diseases, including Griscelli syndrome, choroideremia, and the Hermansky-Pudlak syndrome mouse model, gunmetal. We studied endogenous Rab27a and overexpressed enhanced GFP-Rab27a fusion protein in several cultured melanocyte and melanoma-derived cell lines. In pigmented cells, we observed that Rab27a decorates melanosomes, whereas in nonpigmented cells Rab27a colocalizes with melanosome-resident proteins. When dominant interfering Rab27a mutants were expressedin pigmented cells, we observed a redistribution of pigment granules with perinuclear clustering. This phenotype is similar to that observed by others in melanocytes derived from the ashen and dilute mutant mice, which bear mutations in the Rab27a and MyoVa loci, respectively. We also found that myosinVa coimmunoprecipitates with Rab27a in extracts from melanocytes and that both Rab27a and myosinVa colocalize on the cytoplasmic face of peripheral melanosomes in wildtype melanocytes. However, the amount of myosinVa in melanosomes from Rab27a-deficient ashen melanocytes is greatly reduced. These results, together with recent data implicating myosinVa in the peripheralcapture of melanosomes, suggest that Rab27a is necessary for the recruitment of myosinVa, so allowing the peripheral retention of melanosomes in melanocytes.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/21 alle ore 03:41:20