Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Analysis of expressions of components in the plasminogen activator system in high- and low-metastatic human lung cancer cells
Autore:
He, C; He, P; Liu, LP; Zhu, YS;
Indirizzi:
Shanghai Med Univ, Dept Mol Genet, Shanghai 200032, Peoples R China Shanghai Med Univ Shanghai Peoples R China 200032 00032, Peoples R China Shanghai Med Univ, Liver Canc Inst, Shanghai 200032, Peoples R China Shanghai Med Univ Shanghai Peoples R China 200032 00032, Peoples R China Shanghai Med Univ, Dept Histol & Embryol, Shanghai 200032, Peoples R ChinaShanghai Med Univ Shanghai Peoples R China 200032 00032, Peoples R China
Titolo Testata:
JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY
fascicolo: 3, volume: 127, anno: 2001,
pagine: 180 - 186
SICI:
0171-5216(200103)127:3<180:AOEOCI>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
PRIMARY BREAST-CANCER; UROKINASE RECEPTOR; IN-VITRO; PROGNOSTIC MARKER; INHIBITOR TYPE-1; INVASIVENESS; LINES; PAI-1; UPA; TUMORIGENICITY;
Keywords:
lung cancer; plasminogen activator system; tumor invasion and metastasis;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Zhu, YS Shanghai Med Univ, Dept Mol Genet, Shanghai 200032, Peoples R China Shanghai Med Univ Shanghai Peoples R China 200032 eoples R China
Citazione:
C. He et al., "Analysis of expressions of components in the plasminogen activator system in high- and low-metastatic human lung cancer cells", J CANC RES, 127(3), 2001, pp. 180-186

Abstract

Purpose: To determine the expressive patterns of the components of the plasminogen activator system in human large-cell lung carcinoma strains and toanalyze the effects of the patterns on tumor invasion and metastasis. Methods: The in vitro and in vivo invasive and metastatic potential of two human large-cell lung carcinoma strains with high (strain 95D) and low (strain 95C) metastatic potential was further confirmed by the Boyden chamber modeland nude mice model. After this, the expressions of the components of the plasminogen activator system - including urokinase-type and tissue-type plasminogen activator (uPA and tPA), urokinase receptor (uPAR), and type-1 andtype-2 plasminogen activator inhibitor (PAI-1 and PAI-2) in strain 95D and95C cells were determined by RT-PCR and immunohistochemical staining. The effects of monoclonal antibodies of uPA, uPAR, and PAI-1 on the invasive potential of strain 95D cell line were also evaluated. Results: Strain 95D cells were found to have a stronger in vitro and in vivo invasive and metastatic potential than strain 95C cells. In the former, the average number of infiltrating cells in the in vitro model in one field of vision (40055) was 73.75 +/- 7.42, while in the latter, it was 56.33 +/- 6.28 (P < 0.001). Lung metastatic loci were observed in all six nude mice inoculated with 95D cells (6/ 6), but not in any of the nude mice inoculated with 95C cells (0/6). The high-metastatic strain 95D cells expressed higher uPA and uPAR and lower tPA and PAI-2 than the low-metastatic strain 95C cells. The PAI-1 expressions in both 95D and 95C cells were almost the same. Monoclonal antibodies of uPA and uPAR greatly reduced the invasive potential of strain 95D cells in vitro, Conclusions: These data suggest that the invasive and metastatic potential of human large-cell lung carcinoma cell lines is associated with differential expressions of the components of the plasminogen activator system and that the determination of these components may be used as a marker for judging clinically the possibility of tumor metastasis as well as theprognoses of patients.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/09/20 alle ore 00:44:37