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Titolo:
Selective modulation ef endothelial cell[Ca2+]i response to flow by the onset rate of shear stress
Autore:
Blackman, BR; Thibault, LE; Barbee, KA;
Indirizzi:
Univ Penn, Dept Bioengn, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 t Bioengn, Philadelphia, PA 19104 USA Drexel Univ, Sch Biomed Engn Sci & Hlth Syst, Philadelphia, PA 19104 USA Drexel Univ Philadelphia PA USA 19104 th Syst, Philadelphia, PA 19104 USA
Titolo Testata:
JOURNAL OF BIOMECHANICAL ENGINEERING-TRANSACTIONS OF THE ASME
fascicolo: 3, volume: 122, anno: 2000,
pagine: 274 - 282
SICI:
0148-0731(200006)122:3<274:SMEECR>2.0.ZU;2-L
Fonte:
ISI
Lingua:
ENG
Soggetto:
CAROTID BIFURCATION; CALCIUM TRANSIENTS; TEMPORAL GRADIENT; G-PROTEINS; CELLS; ATHEROSCLEROSIS; ATP; MECHANOTRANSDUCTION; LOCALIZATION; EXPRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Blackman, BR Univ Penn, Dept Bioengn, Philadelphia, PA 19104 USA Univ Penn Philadelphia PA USA 19104 iladelphia, PA 19104 USA
Citazione:
B.R. Blackman et al., "Selective modulation ef endothelial cell[Ca2+]i response to flow by the onset rate of shear stress", J BIOMECH E, 122(3), 2000, pp. 274-282

Abstract

The response of endothelial cells (ECs) to their hemodynamic environment strongly influences normal vascular physiology and the pathogenesis of atherosclerosis. Unique responses to the complex flow patterns in lesion-prone regions imply that the temporal and spatial features of the mechanical stimuli modulate the cellular response to flow. We report the first systematic study of the effects of temporal gradients of shear stress on ECs. Flow was applied to cultured ECs using a novel cone-and-plate device allowing precise and independent control of the shear stress magnitude and the onset rate. Intracellular free calcium concentration ([Ca2+](i)) increased rapidly following the onset of flow, and the characteristics of the transient were modulated by both the shear stress magnitude and onset rate. ECs were most sensitive to shear stress applied at physiological onset rates. Furthermore, the relative contribution of extracellular calcium and IP3-mediated release were dependent upon the specific flow regime.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 11:12:40