Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Targeted site specific cleavage of HIV-1 viral Rev responsive element by copper aminoglycosides
Autore:
Sreedhara, A; Cowan, JA;
Indirizzi:
Ohio State Univ, Evans Lab Chem, Columbus, OH 43210 USA Ohio State Univ Columbus OH USA 43210 ns Lab Chem, Columbus, OH 43210 USA
Titolo Testata:
JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY
fascicolo: 2, volume: 6, anno: 2001,
pagine: 166 - 172
SICI:
0949-8257(200102)6:2<166:TSSCOH>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
A-SITE; RNA; RECOGNITION; INHIBITION; PROTEIN; BINDING; ANTIBIOTICS; RIBOZYME; LIGANDS;
Keywords:
HIV Rev response element RNA; cleavage; aminoglycoside; drug target; copper;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Cowan, JA Ohio State Univ, Evans Lab Chem, 100 W 18th Ave, Columbus, OH 43210 USA Ohio State Univ 100 W 18th Ave Columbus OH USA 43210 H 43210 USA
Citazione:
A. Sreedhara e J.A. Cowan, "Targeted site specific cleavage of HIV-1 viral Rev responsive element by copper aminoglycosides", J BIOL I CH, 6(2), 2001, pp. 166-172

Abstract

Site-specific cleavage of the HIV-1 viral Rev responsive element by copperaminoglycosides is reported under physiological conditions. This bubble and stem-loop RNA structure is efficiently targeted at micromolar concentrations of complex. The specificity of cleavage of structured viral RNA relative to a noncognate tRNA(Phe) of well-defined secondary and tertiary structure is demonstrated. Cleavage products from simpler substrates [diribonucleotide (ApA) and 2',3'-cyclic monophosphate ester (cAMP)] were analyzed by P-31 NMR and demonstrate a hydrolytic mechanism in the absence of external redox agents. These results demonstrate copper aminoglycosides to be highly efficient chemical nucleases with a targeting capability for viral RNA and suggest a novel methodology to counter RNA viruses.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 09:07:58