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Titolo:
Melatonin inhibits the expression of steroidogenic acute regulatory protein and steroidogenesis in MA-10 cells
Autore:
Wu, CS; Leu, SF; Yang, HY; Huang, BM;
Indirizzi:
Natl Cheng Kung Univ, Coll Med, Dept Cell Biol & Anat, Tainan 70101, Taiwan Natl Cheng Kung Univ Tainan Taiwan 70101 ol & Anat, Tainan 70101, Taiwan Natl Cheng Kung Univ, Dept Biol, Tainan 70101, Taiwan Natl Cheng Kung Univ Tainan Taiwan 70101 Dept Biol, Tainan 70101, Taiwan Natl Sci Council, Natl Lab Anim Breeding, Taipei, Taiwan Natl Sci CouncilTaipei Taiwan , Natl Lab Anim Breeding, Taipei, Taiwan Natl Sci Council, Res Ctr, Taipei, Taiwan Natl Sci Council Taipei Taiwan atl Sci Council, Res Ctr, Taipei, Taiwan Natl Taiwan Univ, Dept Zool, Taipei 10764, Taiwan Natl Taiwan Univ Taipei Taiwan 10764 iv, Dept Zool, Taipei 10764, Taiwan
Titolo Testata:
JOURNAL OF ANDROLOGY
fascicolo: 2, volume: 22, anno: 2001,
pagine: 245 - 254
SICI:
0196-3635(200103/04)22:2<245:MITEOS>2.0.ZU;2-M
Fonte:
ISI
Lingua:
ENG
Soggetto:
LEYDIG TUMOR-CELLS; HAMSTERS PHODOPUS-SUNGORUS; MITOCHONDRIAL PROTEINS; LUTEINIZING-HORMONE; IN-VITRO; TESTOSTERONE SYNTHESIS; STIMULATION; RECEPTOR; GONADOTROPIN; ACCUMULATION;
Keywords:
MA-10 Leydig cells; mouse; hCG; P450(SCC); progesterone;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
32
Recensione:
Indirizzi per estratti:
Indirizzo: Huang, BM Natl Cheng Kung Univ, Coll Med, Dept Cell Biol & Anat, Tainan 70101, Taiwan Natl Cheng Kung Univ Tainan Taiwan 70101 Tainan 70101, Taiwan
Citazione:
C.S. Wu et al., "Melatonin inhibits the expression of steroidogenic acute regulatory protein and steroidogenesis in MA-10 cells", J ANDROLOGY, 22(2), 2001, pp. 245-254

Abstract

The effects of melatonin on steroidogenesis and steroidogenic acute regulatory (StAR) protein expression were investigated in MA-IO mouse Leydig tumor cells. MA-10 cells were treated with human chorionic gonadotropin/cyclic adenosine monophosphate (hCG/cAMP) analogue alone or with hCG/cAMP analogueplus melatonin in different dosages (0.1 nM to 10 muM). Steroid productionand the expression of StAR protein were measured. Melatonin directly inhibited hCG- or dbcAMP-stimulated progesterone production in MA-10 cells within 3 hours. The inhibitory effects of melatonin on hCG- or dbcAMP-stimulatedsteroid production in MA-10 cells were abolished by a comparative melatonin receptor antagonist, luzindole. 22R-hydroxycholesterol reversed melatonin's inhibitory effects, which illustrated that melatonin did not suppress P450(scc) enzyme activity. Moreover, StAR protein expression stimulated by hCG and dbcAMP was maximally reduced by 10 nM of melatonin treatment for 3 hours. The effects of prolonged exposure (12 h) to melatonin with dbcAMP stimulation in MA-10 cells were also examined. The expression of StAR protein and steroid production were reduced by melatonin concentrations from 1 nM to10 muM. However, melatonin at a dose of 1 nM had no effect in 3-hour treatment. Our results indicate that melatonin suppressed MA-10 mouse Leydig cell steroidogenesis through specific binding sites by blocking StAR protein expression without altering the activity of P450(scc) enzyme.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/03/20 alle ore 10:46:27