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Titolo:
Proteolytic activation of latent Paraguaya peach PPO. Characterization of monophenolase activity
Autore:
Laveda, F; Nunez-Delicado, E; Garcia-Carmona, F; Sanchez-Ferrer, A;
Indirizzi:
Univ Murcia, Fac Biol, Dept Biochem & Mol Biol A, E-30071 Murcia, Spain Univ Murcia Murcia Spain E-30071 hem & Mol Biol A, E-30071 Murcia, Spain
Titolo Testata:
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
fascicolo: 2, volume: 49, anno: 2001,
pagine: 1003 - 1008
SICI:
0021-8561(200102)49:2<1003:PAOLPP>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
BROAD BEAN POLYPHENOLOXIDASE; DODECYL-SULFATE ACTIVATION; PARTIAL-PURIFICATION; MUSHROOM TYROSINASE; CELL-CULTURES; OXIDASE; SYSTEM;
Keywords:
latent PPO; trypsin; peach; monophenolase activity;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
33
Recensione:
Indirizzi per estratti:
Indirizzo: Sanchez-Ferrer, A Univ Murcia, Fac Vet, Dept Biochem & Mol A, Murcia, Spain Univ Murcia Murcia Spain ochem & Mol A, Murcia, Spain
Citazione:
F. Laveda et al., "Proteolytic activation of latent Paraguaya peach PPO. Characterization of monophenolase activity", J AGR FOOD, 49(2), 2001, pp. 1003-1008

Abstract

The kinetics of the activation process of latent peach PPO by trypsin was studied. By coupling this activation process to the oxidation of 4-tert-butylcatechol (TBC) to its corresponding quinone, it was possible to evaluate the specific rate constant of active PPO formation, k(3), which showed a value of 0.04 s(-1). This proteolytic activation of latent peach PPO permitted us to characterize the monophenolase activity of peach PPO for the first time using p-cresol as substrate, and it showed the characteristic lag period of the kinetic mechanism of monophenols hydroxylation, which depended onthe enzyme and substrate concentration, the pH and the presence of catalytic amounts of o-diphenol (4-methylcatechol). The enzyme activation constant, k(act), was 2 muM.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 01/12/20 alle ore 08:23:44