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Titolo:
Expression of functionally active FcRn and the differentiated bidirectional transport of IgG in human placental endothelial cells
Autore:
Antohe, F; Radulescu, L; Gafencu, A; Ghetie, V; Simionescu, M;
Indirizzi:
Inst Cellular Biol & Pathol N Simionescu, Bucharest 79691, Romania Inst Cellular Biol & Pathol N Simionescu Bucharest Romania 79691 Romania Univ Texas, SW Med Ctr, Dallas, TX 75230 USA Univ Texas Dallas TX USA 75230 iv Texas, SW Med Ctr, Dallas, TX 75230 USA
Titolo Testata:
HUMAN IMMUNOLOGY
fascicolo: 2, volume: 62, anno: 2001,
pagine: 93 - 105
SICI:
0198-8859(200102)62:2<93:EOFAFA>2.0.ZU;2-Q
Fonte:
ISI
Lingua:
ENG
Soggetto:
CANINE KIDNEY-CELLS; I-RELATED RECEPTOR; IMMUNOGLOBULIN-G; COMPLEX; MICE; TROPHOBLAST; SYNCYTIOTROPHOBLAST; PERMEABILITY; TRANSCYTOSIS; ENDOCYTOSIS;
Keywords:
human IgG; FcRn; transcytosis; endothelial cells; placenta;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
37
Recensione:
Indirizzi per estratti:
Indirizzo: Simionescu, M Inst Cellular Biol & Pathol N Simionescu, 8 BP Hasdeu St,POB35-14, Bucharest 79691, Romania Inst Cellular Biol & Pathol N Simionescu 8BP Hasdeu St,POB 35-14 Bucharest Romania 79691
Citazione:
F. Antohe et al., "Expression of functionally active FcRn and the differentiated bidirectional transport of IgG in human placental endothelial cells", HUMAN IMMUN, 62(2), 2001, pp. 93-105

Abstract

The mechanism of selective transport of the immunoglobulins G from the placental struma to che lumen of the fetal blood vessels has not been elucidated yet. It was postulated that the specific transport as well as the regulation of IgG level ill the blood, involves the MHC class I related receptor FcRn for the Fc domain of IgG. We questioned whether human placental endothelial cells (HPEC) express FcRn and, if present, whether it is in a functionally active form. The experiments were performed on cultured HPEC and as positive control, human trophoblastic (JEG3) and mouse endothelial cells (SVEC) were used. Expression of FcRn, was demonstrated by indirect immunofluorescence and RT-PCR. The role of FcRn was assessed by quantifying the transcellular transport of [I-125]-hIgG or [I-125)-rF(ab')(2) fragments from the apical to basolateral surface, and in the reverse direction of HPEC grown on filters in a double chamber system. The intracellular pathway of FcRn or IgG was examined by electron microscopy using the proteins adsorbed to 5 nmand 20 nm colloidal Sold particles, respectively. The results showed that:(a) FcRn is expressed by human placental endothelial cells, in a functionally active form; ib) transcytosis of IgG in HPEC is a rime-dependent process that cakes place preferentially from the basolateral to the apical compartment; and (c) both IgG and FcRn colocalize in an intracellular endocytic compartment, chloroquine sensitive. Together these data suggest that the regulation of IgG level by endothelial cells may result from interplay betweensalvaging, exocytosis, and transcytosis of the molecules. One can assume that IgG that does not bind to FcRn may be destined For destruction, and this would explain the mechanism by which IgG homeostasis is maintained. (C) American Society for Histocompatibility and Immunogenetics, 2001. Published by Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 30/11/20 alle ore 09:23:38