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Titolo:
Monoallelic expresion of the odourant receptor gene and axonal projection of olfactory sensory neurones
Autore:
Ishii, T; Serizawa, S; Kohda, A; Nakatani, H; Shiroishi, T; Okumura, K; Iwakura, Y; Nagawa, F; Tsuboi, A; Sakano, H;
Indirizzi:
Univ Tokyo, Grad Sch Sci, Dept Biophys & Biochem, Tokyo 1130032, Japan Univ Tokyo Tokyo Japan 1130032 t Biophys & Biochem, Tokyo 1130032, Japan Mie Univ, Fac Bioresources, Mol & Cellular Biol Lab, Tsu, Mie 5148507, Japan Mie Univ Tsu Mie Japan 5148507 Cellular Biol Lab, Tsu, Mie 5148507, Japan Natl Inst Genet, Mammalian Genet Lab, Mishima, Shizuoka 4110801, Japan Natl Inst Genet Mishima Shizuoka Japan 4110801 a, Shizuoka 4110801, Japan Univ Tokyo, Inst Med Sci, Ctr Med Expt, Tokyo 1088639, Japan Univ Tokyo Tokyo Japan 1088639 d Sci, Ctr Med Expt, Tokyo 1088639, Japan Japan Sci & Technol Corp, PRESTO, Kumamoto 8600012, Japan Japan Sci & Technol Corp Kumamoto Japan 8600012 Kumamoto 8600012, Japan
Titolo Testata:
GENES TO CELLS
fascicolo: 1, volume: 6, anno: 2001,
pagine: 71 - 78
SICI:
1356-9597(200101)6:1<71:MEOTOR>2.0.ZU;2-1
Fonte:
ISI
Lingua:
ENG
Soggetto:
ODORANT RECEPTOR; FUNCTIONAL EXPRESSION; BULB; MAP; IDENTIFICATION; ORGANIZATION; EPITHELIUM;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
18
Recensione:
Indirizzi per estratti:
Indirizzo: Sakano, H Univ Tokyo, Grad Sch Sci, Dept Biophys & Biochem, Tokyo 1130032,Japan Univ Tokyo Tokyo Japan 1130032 & Biochem, Tokyo 1130032, Japan
Citazione:
T. Ishii et al., "Monoallelic expresion of the odourant receptor gene and axonal projection of olfactory sensory neurones", GENES CELLS, 6(1), 2001, pp. 71-78

Abstract

Background: We have previously generated transgenic mice carrying the murine odourant receptor gene, MOR28, tagged with lacZ. In this animal, the endogenous MOR28 is differently tagged with GFP. It was found that the transgenic and endogenous MOR28 genes are expressed in a mutually exclusive mannerand that the two sets of olfactory sensory neurones (OSNs), each expressing either the transgenic or the endogenous MOR28, project their axons to separate glomeruli. Results: Our fluorescent in situ hybridization (FISH) revealed that the two endogenous alleles of MOR28 are also mutually excluded for their transcriptional activation. Therefore, we studied whether there would be any segregation in the projection of the two subsets of OSNs: one set expressing the paternal and the other expressing the maternal allele. It was found that the OSNs for both alleles shared the same glomerulus for their projection, but the projection targets were segregated within the glomerular structure. Conclusion: Two subsets of neurones expressing either the transgenic or the endogenous MOR28 target their axons to two separate glomeruli based on the differences in the genetic backgrounds, nature of tagging, and chromosomal locations. In contrast, neurones expressing a maternal or paternal alleleshare the same glomeruli, but tend to target to segregated areas within the glomerular structure. The segregation was more prominent with increased differences in the genetic background between the two alleles.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 12/07/20 alle ore 11:57:06