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Titolo:
Cloning of a novel isoform of the mouse NBMPR-sensitive equilibrative nucleoside transporter (ENT1) lacking a putative phosphorylation site
Autore:
Handa, M; Choi, DS; Caldeiro, RM; Messing, RO; Gordon, AS; Diamond, I;
Indirizzi:
Univ Calif San Francisco, Ernest Gallo Clin & Res Ctr, Dept Neurol, Emeryville, CA 94608 USA Univ Calif San Francisco Emeryville CA USA 94608 Emeryville, CA 94608 USA Univ Calif San Francisco, Grad Program Neurosci, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Univ Calif San Francisco, Wheeler Ctr Neurobiol Addict, San Francisco, CA 94143 USA Univ Calif San Francisco San Francisco CA USA 94143 ancisco, CA 94143 USA Univ Calif San Francisco, Dept Mol & Cellular Pharmacol, San Francisco, CA94143 USA Univ Calif San Francisco San Francisco CA USA 94143 rancisco, CA94143 USA
Titolo Testata:
GENE
fascicolo: 1-2, volume: 262, anno: 2001,
pagine: 301 - 307
SICI:
0378-1119(20010110)262:1-2<301:COANIO>2.0.ZU;2-9
Fonte:
ISI
Lingua:
ENG
Soggetto:
ADENOSINERGIC MODULATION; MOLECULAR-BIOLOGY; ETHANOL; EI;
Keywords:
adenosine; NBMPR-sensitive equilibrative nucleoside transporter; phosphorylation site;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
20
Recensione:
Indirizzi per estratti:
Indirizzo: Diamond, I Univ Calif San Francisco, Ernest Gallo Clin & Res Ctr, Dept Neurol, 5858 Horton St,Suite 200, Emeryville, CA 94608 USA Univ Calif San Francisco 5858 Horton St,Suite 200 Emeryville CA USA 94608
Citazione:
M. Handa et al., "Cloning of a novel isoform of the mouse NBMPR-sensitive equilibrative nucleoside transporter (ENT1) lacking a putative phosphorylation site", GENE, 262(1-2), 2001, pp. 301-307

Abstract

We have isolated a mouse cDNA clone corresponding to a novel isoform of the NBMPR-sensitive equilibrative nucleoside transporter (ENT1). The cDNA contains a 6 bp deletion in the open reading frame that changes the amino acidcomposition in a consensus casein kinase II (CKII) phosphorylation site atSer-254. The clone containing Ser-254 is termed mENT1.1 and the clone lacking the serine termed mENT1.2. The deduced amino acid sequence of mENT1.1 corresponds to the previously cloned human and rat ENT1 proteins at Ser-254. Tissue distribution studies show that mRNA for both ENT1 isoforms are ubiquitously co-expressed in mouse. Analysis of genomic DNA corresponding to mouse ENT1 indicates the isoforms can be produced by alternative splicing at the end of exon 7. CEM/C19 cells stably expressing mENT1.1 and mENT1.2 showsimilar dose response curves for NBMPR and dipyridamole inhibition of [H-3]adenosine uptake as well as exhibiting comparable selectivity for both purine and pyrimidine nucleosides but not the corresponding nucleobases. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 20:34:24