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Titolo:
Gene structure, chromosomal localization and immunolocalization of chickencentromere proteins CENP-C and ZW10
Autore:
Okamura, A; Pendon, C; Valdivia, MM; Ikemura, T; Fukagawa, T;
Indirizzi:
Natl Inst Genet, Dept Evolutionary Genet, Mishima, Shizuoka 4118540, JapanNatl Inst Genet Mishima Shizuoka Japan 4118540 a, Shizuoka 4118540, Japan Grad Univ Adv Studies, Mishima, Shizuoka 4118540, Japan Grad Univ Adv Studies Mishima Shizuoka Japan 4118540 zuoka 4118540, Japan Univ Cadiz, Dept Biochem & Mol Biol, Cadiz 11510, Spain Univ Cadiz CadizSpain 11510 Dept Biochem & Mol Biol, Cadiz 11510, Spain
Titolo Testata:
GENE
fascicolo: 1-2, volume: 262, anno: 2001,
pagine: 283 - 290
SICI:
0378-1119(20010110)262:1-2<283:GSCLAI>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
KINETOCHORE; IDENTIFICATION; SCLERODERMA; ANAPHASE; DOMAINS;
Keywords:
centromere; DT40; genomic structure; ZW10; CENP-C;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Fukagawa, T Natl Inst Genet, Dept Evolutionary Genet, Mishima, Shizuoka 4118540, Japan Natl Inst Genet Mishima Shizuoka Japan 4118540 4118540, Japan
Citazione:
A. Okamura et al., "Gene structure, chromosomal localization and immunolocalization of chickencentromere proteins CENP-C and ZW10", GENE, 262(1-2), 2001, pp. 283-290

Abstract

We determined the genomic structures and complete sequences of the coding regions of the chicken CENP-C and ZW10 genes. These two genes encode proteins that are thought to be involved in maintaining the fidelity of chromosome segregation. The chicken CENP-C gene is 30 kb in length and contains 19 exons. The chicken ZW10 gene spans 10 kb and contains 15 exons. The 5'-untranslated regions of these genes contain several binding sites for transcription factors such as Sp-1, E2F, p300, and members of the GATA family. By fluorescence in situ hybridization (FISH) analysis, the CENP-C was mapped to chromosome 4 and the ZW10 gene was: mapped to a microchromosome. Antibodies against the chicken ZW10 protein revealed a cell cycle-dependent staining pattern in DT40 cells. ZW10 protein was distributed throughout the cytoplasmof DT40 cells during interphase. In most metaphase cells, ZW10 proteins appeared equally divided between the centromere and the spindle apparatus. During anaphase, chicken ZW10 proteins were no longer localized near chromosomes or the mitotic apparatus but were present diffusely in the cytoplasm. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 20/01/21 alle ore 12:30:23