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Titolo:
Expression of 12-halobenzoate dioxygenase genes (cbdSABC) involved in the degradation of benzoate and 2-halobenzoate in Burkholderia sp TH2
Autore:
Suzuki, K; Ogawa, N; Miyashita, K;
Indirizzi:
Natl Inst Agroenvironm Sci, Tsukuba, Ibaraki 3058604, Japan Natl Inst Agroenvironm Sci Tsukuba Ibaraki Japan 3058604 i 3058604, Japan
Titolo Testata:
GENE
fascicolo: 1-2, volume: 262, anno: 2001,
pagine: 137 - 145
SICI:
0378-1119(20010110)262:1-2<137:EO1DG(>2.0.ZU;2-#
Fonte:
ISI
Lingua:
ENG
Soggetto:
CLEAVAGE PATHWAY OPERON; PSEUDOMONAS-CEPACIA 2CBS; TOL PLASMID; POSITIVE REGULATOR; ESCHERICHIA-COLI; XYLS; TRANSCRIPTION; STREPTOMYCES; PUTIDA; 1,2-DIOXYGENASE;
Keywords:
2-halobenzoate dioxygenase; transcriptional activator;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
30
Recensione:
Indirizzi per estratti:
Indirizzo: Suzuki, K Natl Inst Agroenvironm Sci, 3-1-1 Kan Nondai, Tsukuba, Ibaraki 3058604, Japan Natl Inst Agroenvironm Sci 3-1-1 Kan Nondai Tsukuba Ibaraki Japan 3058604
Citazione:
K. Suzuki et al., "Expression of 12-halobenzoate dioxygenase genes (cbdSABC) involved in the degradation of benzoate and 2-halobenzoate in Burkholderia sp TH2", GENE, 262(1-2), 2001, pp. 137-145

Abstract

Burkholderia sp. TH2, isolated from soil, utilizes 2-chlorobenzoate (2CB) and benzoate (BA) as its sole source of carbon and energy. The genes for 2-halobenzoate dioxygenase (cbdABC) from Burkholderia sp. TH2 were cloned andsequenced. The predicted amino acid sequences of all the gene products arehighly similar to the cbd gene products of Pseudomonas sp. 2CBS. Disruption of the promoter region of cbdA resulted in loss of growth on 2CB and BA, indicating that these genes are involved in the growth of TH2 on these substrates. Expression of the cbd genes was analyzed by transcriptional fusion assay. The cbdS gene, a possible araC/xylS-type transcriptional regulatory gene, was shown to positively regulate the expression of cbdA. In addition,the effectors of CbdS were shown to be 2CB, 2-bromobenzoate, o-toluate (2-methylbenzoate), 2-iodobenzoate, and BA. Primer extension analysis showed that the cbdA mRNA started at two positions, 14 and 15 nucleotides upstream from the cbdA start codon, ATG. A pail of direct repeats, identical to thatof the Pin promoter of the TOL plasmid, was found upstream of -35 hexamer of the cbdA promoter. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/09/20 alle ore 12:49:44