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Titolo:
Modulation of the promoter region of prepro-hypocretin by alpha-interferon
Autore:
Waleh, NS; Apte-Deshpande, A; Terao, A; Ding, J; Kilduff, TS;
Indirizzi:
SRI Int, Menlo Park, CA 94025 USA SRI Int Menlo Park CA USA 94025SRI Int, Menlo Park, CA 94025 USA
Titolo Testata:
GENE
fascicolo: 1-2, volume: 262, anno: 2001,
pagine: 123 - 128
SICI:
0378-1119(20010110)262:1-2<123:MOTPRO>2.0.ZU;2-X
Fonte:
ISI
Lingua:
ENG
Soggetto:
TRANSCRIPTION FACTOR; FEEDING-BEHAVIOR; HUMAN NARCOLEPSY; GENE-EXPRESSION; OBESE GENE; OREXIN-A; SLEEP; PROTEIN; NEURONS; PEPTIDES;
Keywords:
prepro-orexin; binding site; transcription factor; alpha-interferon;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
39
Recensione:
Indirizzi per estratti:
Indirizzo: Waleh, NS SRI Int, 333 Ravenswood Ave, Menlo Park, CA 94025 USA SRI Int 333 Ravenswood Ave Menlo Park CA USA 94025 CA 94025 USA
Citazione:
N.S. Waleh et al., "Modulation of the promoter region of prepro-hypocretin by alpha-interferon", GENE, 262(1-2), 2001, pp. 123-128

Abstract

Hypocretins 1 and 2 (also called orexins A and B, respectively) are hypothalamic neuropeptides that have recently been shown to be involved in the sleep disorder narcolepsy and possibly in the normal regulation of sleep and wake functions. These two peptides are derived from a single precursor molecule called prepro-hypocretin, also known as prepro-orexin. We have cloned a 450 bp fragment from the 5'-flanking region of the human prepro-hypocretin gene and demonstrated that this fragment has promoter activity in vitro. Deletions at the 5' end from -450 to -188 reduced the promoter activity by similar to 50%. Further deletion from the 5'-end to -69 almost completely abolished promoter activity. The 450 bp fragment contains a number of potential transcription factor binding sites, including an interferon (IFN) response element. Our studies: demonstrate that alpha -IFN strongly inhibits thepromoter activity of both 450 and 188 bp fragments in a dose-dependent manner. The inhibitory effect of alpha -IFN is consistent with recent studies which suggest that hypocretin 1/orexin A may be involved in modulating arousal states and with the literature indicating involvement of immune-relatedmolecules in sleep regulation. (C) 2001 Elsevier Science B.V. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 12:17:36