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Titolo:
In vivo evidence for accelerated generation of hydroxyl radicals in liver of Long-Evans Cinnamon (LEC) rats with acute hepatitis
Autore:
Yamamoto, H; Watanabe, T; Mizuno, H; Endo, K; Hosokawa, T; Kazusaka, A; Gooneratne, R; Fujita, S;
Indirizzi:
Hokkaido Univ, Grad Sch Vet Med, Dept Environm Vet Sci, Lab Toxicol,Kita Ku, Sapporo, Hokkaido 0600818, Japan Hokkaido Univ Sapporo Hokkaido Japan 0600818 oro, Hokkaido 0600818, Japan Sankyo Co Ltd, Med Safety Res Labs, Tokyo 140, Japan Sankyo Co Ltd TokyoJapan 140 Ltd, Med Safety Res Labs, Tokyo 140, Japan Lincoln Univ, Anim & Food Sci Div, Canterbury, New Zealand Lincoln Univ Canterbury New Zealand od Sci Div, Canterbury, New Zealand
Titolo Testata:
FREE RADICAL BIOLOGY AND MEDICINE
fascicolo: 5, volume: 30, anno: 2001,
pagine: 547 - 554
SICI:
0891-5849(20010301)30:5<547:IVEFAG>2.0.ZU;2-Z
Fonte:
ISI
Lingua:
ENG
Soggetto:
WILSONS-DISEASE; COPPER-METALLOTHIONEIN; HEREDITARY HEPATITIS; MECHANISTIC ASPECTS; LIPID-PEROXIDATION; TRANSITION-METALS; HYDROGEN-PEROXIDE; BILIARY-EXCRETION; SALICYLATE; INJURY;
Keywords:
LEC rats; copper; hepatitis; hydroxyl radicals; salicylic acid; mannitol; free radicals;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
46
Recensione:
Indirizzi per estratti:
Indirizzo: Fujita, S Hokkaido Univ, Grad Sch Vet Med, Dept Environm Vet Sci, Lab Toxicol,Kita Ku, N18,W9, Sapporo, Hokkaido 0600818, Japan Hokkaido Univ N18,W9 Sapporo Hokkaido Japan 0600818 00818, Japan
Citazione:
H. Yamamoto et al., "In vivo evidence for accelerated generation of hydroxyl radicals in liver of Long-Evans Cinnamon (LEC) rats with acute hepatitis", FREE RAD B, 30(5), 2001, pp. 547-554

Abstract

The Long-Evans Cinnamon (LEC) rats accumulate excess copper (Cu) in the liver in a manner similar to patients with Wilson's disease (WD) and spontaneously develop acute hepatitis with severe jaundice. Although hydroxyl radicals ((OH)-O-.) have been proposed to be a cause of hepatitis by the accumulation of Cu, it is not clear whether or not (OH)-O-. can be produced in theLiver of hepatitic LEC rats in vivo and also can be involved in the onset of hepatitis. In the present study,(OH)-O-. production in plasma and Liver of hepatitic LEC rats was quantified by trapping (OH)-O-. with salicylic acid (SA) as 2, 3-dihydroxybenzoic acid (2, 3-DHBA). The ratios of 2, 3-DHBA/SA were significantly higher in plasma and liver of hepatitic LEC rats thanthose of Wistar rats and LEC rats showing no signs of hepatitis. Furthermore, the ratios of 2, 3-DHBA/SA in plasma and liver of hepatitic LEC rats were almost the same as those of Wistar rats treated orally with CuSO4 (0.5 mmol/kg) 2 h before acetylsalicylic acid (ASA) injection. We also evaluated the protective effects of D-mannitol (a (OH)-O-. scavenger) treatment against acute hepatitis in LEC rats. D-mannitol (500 mg/kg) was administered intraperitoneally to 10-week-old LEC rats for 3 weeks. D-mannitol treatment suppressed the increases in serum aspartate aminotransferase activity and total bilirubin concentration. In addition, D-mannitol treatment significantlyreduced hepatic mitochondrial lipid peroxidation, which is thought to be important in the pathogenesis of Cu-induced hepatotoxicity. These observations suggest that accelerated generation of (OH)-O-. catalyzed by free Cu in the Liver may, at least in part, play a role in the pathogenesis of acute hepatitis in LEC rats. (C) 2001 Elsevier Science Inc.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/04/20 alle ore 03:55:39