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Titolo:
Crithidia guilhermei: Purification and partial characterization of a 62-kDa extracellular metalloproteinase
Autore:
de Melo, ACN; Giovanni-De-Simone, S; Branquinha, MH; Vermelho, AB;
Indirizzi:
Univ Fed Rio de Janeiro, Inst Microbiol Prof Paulo de Gooes, Dept Gen Microbiol, CCS,BI I, BR-21941590 Rio De Janeiro, Brazil Univ Fed Rio de Janeiro Rio De Janeiro Brazil BR-21941590 BCeiro, Brazil Univ Fed Fluminense, Dept Mol & Cellular Biol, Niteroi, RJ, Brazil Univ Fed Fluminense Niteroi RJ Brazil Cellular Biol, Niteroi, RJ, Brazil Inst Oswaldo Cruz, Dept Biochem & Mol Biol, BR-20001 Rio De Janeiro, Brazil Inst Oswaldo Cruz Rio De Janeiro Brazil BR-20001 Rio De Janeiro, Brazil
Titolo Testata:
EXPERIMENTAL PARASITOLOGY
fascicolo: 1, volume: 97, anno: 2001,
pagine: 1 - 8
SICI:
0014-4894(200101)97:1<1:CGPAPC>2.0.ZU;2-3
Fonte:
ISI
Lingua:
ENG
Soggetto:
PROMASTIGOTE SURFACE PROTEASE; TRYPANOSOMA-CRUZI; LEISHMANIA-MEXICANA; CYSTEINE PROTEINASE; GP63; IDENTIFICATION; ENDOPEPTIDASE; PATHOGENICITY; INHIBITORS; CUPRINA;
Keywords:
Crithidia guilhermei; trypanosomatid; purification; extracellular proteinase; metalloproteinase; gelatin; ammonium sulfate [(NH4)(2)SO4]; sodium dodecyl sulfate-polyacrylamide gel electrophoresis; soybean trypsin inhibitor; ethylenediaminetetraacetic acid; phenymethysulfonyl fluoride; trans-epoxysuccinyl-L-leucylamido(4-guanidino) butane; zinc sulfate; calcium chloride; high-performance liquid chromatography; promastigote surface proteinase;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Vermelho, AB Univ Fed Rio de Janeiro, Inst Microbiol Prof Paulo de Gooes, Dept Gen Microbiol, CCS,BI I, BR-21941590 Rio De Janeiro, Brazil Univ Fed Rio de Janeiro Rio De Janeiro Brazil BR-21941590 BC
Citazione:
A.C.N. de Melo et al., "Crithidia guilhermei: Purification and partial characterization of a 62-kDa extracellular metalloproteinase", EXP PARASIT, 97(1), 2001, pp. 1-8

Abstract

An extracellular metalloproteinase from Crithidia guilhermei, a monoxenic trypanosomatid of insects, was purified 11-fold by ammonium sulfate precipitation, gel filtration on a Shinpack Diol-150 column, and anion-exchange chromatography in a MONO Q column, both using the HPLC system. The proteinaseappeared as a single band with an apparent molecular mass of 62 kDa in SDS-PAGE, under reducing conditions, and was optimally active at 37 degreesC and pH 6.0. The enzyme showed 62% residual activity at 50 degreesC for 30 min. The proteinase was completely inhibited by 1, 10-phenanthroline, indicating that the enzyme belongs to the metalloproteinase class. This is the first report of the purification of an extracellular metalloproteinase from the Crithidia species. The possible role of this enzyme in the digestive tract of the insect host is discussed. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 27/11/20 alle ore 06:34:39