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Titolo:
Contrasting effects of nitric oxide on food intake and GH secretion stimulated by a GH-releasing peptide
Autore:
Rigamonti, AE; Cella, SG; Cavallera, GM; Deghenghi, R; Locatelli, V; Pitsikas, N; Muller, EE;
Indirizzi:
Univ Milan, Dept Med Pharmacol, I-20129 Milan, Italy Univ Milan Milan Italy I-20129 Dept Med Pharmacol, I-20129 Milan, Italy Europeptides, Argenteuil, France Europeptides Argenteuil FranceEuropeptides, Argenteuil, France Boehringer Ingelheim Italia SPA, Milan, Italy Boehringer Ingelheim Italia SPA Milan Italy im Italia SPA, Milan, Italy
Titolo Testata:
EUROPEAN JOURNAL OF ENDOCRINOLOGY
fascicolo: 2, volume: 144, anno: 2001,
pagine: 155 - 162
SICI:
0804-4643(200102)144:2<155:CEONOO>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
GROWTH-HORMONE SECRETION; ARGININE METHYL-ESTER; COMPETITIVE ANTAGONISM; PITUITARY-GLAND; MESSENGER-RNA; LH-SECRETION; NORMAL MEN; IN-VITRO; SYNTHASE; RATS;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
57
Recensione:
Indirizzi per estratti:
Indirizzo: Muller, EE Univ Milan, Dept Med Pharmacol, Via Vanvitelli 32, I-20129 Milan, Italy Univ Milan Via Vanvitelli 32 Milan Italy I-20129 Milan, Italy
Citazione:
A.E. Rigamonti et al., "Contrasting effects of nitric oxide on food intake and GH secretion stimulated by a GH-releasing peptide", EUR J ENDOC, 144(2), 2001, pp. 155-162

Abstract

Objective: Among the many actions of nitric oxide (NO) are those on endocrine and feeding behaviour. Based on NO involvement in the GH-releasing effect of the CH-releasing peptides (GHRPs) and the reported orexigenic activity of these compounds, we sought to evaluate the effect of the combined administration of a long-acting NO donor, molsidomine, and the newly synthesized GHRP EP92632 on food intake and GH secretion in rats. Moreover, to verifythe specificity of a potential NO involvement, we evaluated whether or notthe effects of GHRPs were abolished by a pre-treatment with an inhibitor of NO synthase, N-nitro-arginine-methyl-ester (NAME). Methods: In the food intake experiments, adult Sprague-Dawley male rats underwent acute administration of: (1) EP92632 (160 mug/kg. s.c.): (2) molsidomine (100 mg/kg, i.p.); (3) EP92632+molsidomine: (4) L-NAME (40 and 60 mg/kg, i.p,): (5) EP92632+L-NAME (60 mg/kg, i.p.): (6) EP92632+molsidomine+L-NAME (60 mg/kg, i.p.): and (7) 0.9%, saline (0.1 ml/kg, i.p.). After treatments, the cumulative food intake in the 6 post-treatment hours was carefullyevaluated. In the neuroendocrine experiments, rats were given the same compounds according to the above reported schedule, except for the use of one dose of NAME (60 mg/kg, i.p.) and a lower EP92632 dose (80 mug/kg. s.c.), and were sampled via atrial cannula. Results: EP92632 significantly stimulated food intake, an effect which wasfurther enhanced by molsidomine, though the latter did not elicit per se any orexigenic effect. L-NAME given alone significantly decreased food intake and abolished the orexigenic effect of the GHRP and the enhancing effect of molsidomine. Plasma GH levels increased significantly following administration of EP92632 but, in contrast to the food intake experiments, molsidomine significantly inhibited both basal and EP92632-stimulated GH secretion:moreover, NAME had a biphasic effect on the EP92632-stimulated GH release:initially inhibitory and then, from 45 min on, stimulatory. NAME did not affect basal GH levels but, surprisingly, combined administration of molsidomine and NAME induced a striking inhibition of both basal and the peptide-stimulated GH release. Conclusions: In summary. these data indicate that NO in the rat is physiologically involved in a stimulatory way in the GHRP-mediated effect on food intake, but exerts a dual action, probably stimulatory at hypothalamic and inhibitory at pituitary levels, on basal and GHRP-stimulated CH secretion.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 04/04/20 alle ore 07:59:32