Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Effects of semiconductor substrate and glia-free culture on the development of voltage-dependent currents in rat striatal neurones
Autore:
Parak, WJ; George, M; Kudera, R; Gaub, HE; Behrends, JC;
Indirizzi:
Univ Munich, Ctr Nanosci, D-80336 Munich, Germany Univ Munich Munich Germany D-80336 Ctr Nanosci, D-80336 Munich, Germany Univ Munich, Dept Physiol, D-80336 Munich, Germany Univ Munich Munich Germany D-80336 Dept Physiol, D-80336 Munich, Germany Univ Munich, Ctr Nanosci, D-80799 Munich, Germany Univ Munich Munich Germany D-80799 Ctr Nanosci, D-80799 Munich, Germany Univ Munich, Dept Appl Phys & Biophys, D-80799 Munich, Germany Univ Munich Munich Germany D-80799 ys & Biophys, D-80799 Munich, Germany
Titolo Testata:
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
fascicolo: 8, volume: 29, anno: 2001,
pagine: 607 - 620
SICI:
0175-7571(2001)29:8<607:EOSSAG>2.0.ZU;2-S
Fonte:
ISI
Lingua:
ENG
Soggetto:
SERUM-FREE MEDIUM; LAMININ GAMMA-1 CHAIN; GROWTH CONE GUIDANCE; SENSORY NERVE-FIBERS; NIDOGEN BINDING-SITE; HIPPOCAMPAL-NEURONS; MAMMALIAN-CELLS; THIN-FILMS; IN-VITRO; SURFACE-CHEMISTRY;
Keywords:
semiconductor; culture; development; inward current; outward current;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
75
Recensione:
Indirizzi per estratti:
Indirizzo: Behrends, JC Univ Munich, Ctr Nanosci, Pettenkoferstr 12, D-80336 Munich, Germany Univ Munich Pettenkoferstr 12 Munich Germany D-80336 Germany
Citazione:
W.J. Parak et al., "Effects of semiconductor substrate and glia-free culture on the development of voltage-dependent currents in rat striatal neurones", EUR BIOPHYS, 29(8), 2001, pp. 607-620

Abstract

An essential requirement for successful longterm coupling between neuronalassemblies and semiconductor devices is that the neurones must be able to fully develop their electrogenic repertoire when growing on semiconductor (silicon) substrates. While it has for some time been known that neurones may be cultured on silicon wafers insulated with SiO2 and Si3N4, an electrophysiological characterisation of their development under such conditions is lacking. The development of voltage-dependent membrane currents, especiallyof the rapid sodium inward current underlying the action potential, is of particular importance because the conductance change during the action potential determines the quality of cell-semiconductor coupling. We have cultured rat striatal neurones on either glass coverslips or silicon wafers insulated with SiO2 and Si3N4 using both serum-containing and serum-free media. We here report evidence that not only serum-free culture media but also growth on semiconductor surfaces may negatively affect the development of voltage-dependent currents in neurones. Furthermore, using surface-charge measurements with the atomic force microscope, we demonstrate a reduced negativity of the semiconductor surface compared to glass. The reduced surface charge may affect cellular development through an effect on the binding and/or orientation of extracellular matrix proteins, such as laminin. Our findingstherefore suggest that semiconductor substrates are not entirely equivalent to glass in terms of their effects on neuronal cell growth and differentiation.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 14/07/20 alle ore 06:46:10