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Titolo:
PCR techniques for clonality assays
Autore:
Diaz-Cano, SJ; Blanes, A; Wolfe, HJ;
Indirizzi:
Tufts Univ, New England Med Ctr, Dept Pathol, Boston, MA 02111 USA Tufts Univ Boston MA USA 02111 Med Ctr, Dept Pathol, Boston, MA 02111 USA St Bartholomews & Royal London Sch Med & Den, London, England St Bartholomews & Royal London Sch Med & Den London England on, England Univ Malaga, E-29071 Malaga, Spain Univ Malaga Malaga Spain E-29071Univ Malaga, E-29071 Malaga, Spain
Titolo Testata:
DIAGNOSTIC MOLECULAR PATHOLOGY
fascicolo: 1, volume: 10, anno: 2001,
pagine: 24 - 33
SICI:
1052-9551(200103)10:1<24:PTFCA>2.0.ZU;2-2
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYMERASE-CHAIN-REACTION; MICROSATELLITE INSTABILITY; INTRATUMOR HETEROGENEITY; CHROMOSOME INACTIVATION; GENETIC-HETEROGENEITY; REPEAT POLYMORPHISM; CELL CARCINOMA; BREAST-CANCER; K-RAS; TUMORS;
Keywords:
clonality; X chromosome inactivation; microsatellites; tumor suppressor genes; tumor progression; paraffin-embedded tissues;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
61
Recensione:
Indirizzi per estratti:
Indirizzo: Diaz-Cano, SJ St Bartholomews & London NHS Trust, Dept Histopathol & Morbid Anat, LondonE1 1BB, England St Bartholomews & London NHS Trust London England E1 1BB d
Citazione:
S.J. Diaz-Cano et al., "PCR techniques for clonality assays", DIAGN MOL P, 10(1), 2001, pp. 24-33

Abstract

Clonal overgrowths represent the hallmark of neoplastic proliferations, and their demonstration has been proved useful clinically for the diagnosis of malignant lymphomas based on the detection of specific and dominant immunoglobulin and/or T-cell receptor gene rearrangements. Nonrandom genetic alterations can also be used to test clonal expansions and the clonal evolution of neoplasms, especially analyzing hypervariable deoxyribonucleic acid (DNA) regions from patients heterozygous for a given marker. These tests relybasically on the demonstration of loss of heterozygosity (LOH) resulting from either hemizygosity (nonrandom interstitial DNA deletions) or homozygosity of mutant alleles observed in neoplasms. LOH analyses identify clonal expansions of a tumor cell population, and point to monoclonal proliferationwhen multiple and consistent LOH are demonstrated. Based on the methylation-related inactivation of one X chromosome in female subjects, X-linked markers (e.g,, androgen receptor gene) will provide clonality information using LOH analyses after DNA digestion with methylation-sensitive restriction endonucleases. Therefore, both non-X-linked and X-linked analyses give complementary information, related and not related to the malignant transformation pathway respectively. Applied appropriately, these tools can establish the clonal evolution of tumor cell populations (tumor heterogeneity), identify early relapses, distinguish recurrent tumors from other metachronic neoplasms, and differentiate field transformation from metastatic tumor growthsin synchronic and histologically identical neoplasms.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 16/07/20 alle ore 19:54:30