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Titolo:
Translocation (10;11;22)(p14;q24;q12) characterized by fluorescence in situ hybridization in a case of Ewing's tumor
Autore:
Noguera, R; Pellin, A; Navarro, S; Carda, C; Llombart-Bosch, A;
Indirizzi:
Univ Valencia, Sch Med, Dept Pathol, Valencia 46010, Spain Univ Valencia Valencia Spain 46010 d, Dept Pathol, Valencia 46010, Spain
Titolo Testata:
DIAGNOSTIC MOLECULAR PATHOLOGY
fascicolo: 1, volume: 10, anno: 2001,
pagine: 2 - 8
SICI:
1052-9551(200103)10:1<2:T(CBFI>2.0.ZU;2-U
Fonte:
ISI
Lingua:
ENG
Soggetto:
ROUND-CELL TUMORS; PERIPHERAL NEUROECTODERMAL TUMOR; PARAFFIN-EMBEDDED TISSUE; SOFT-TISSUE; CYTOGENETIC ANALYSIS; EWS GENE; CHROMOSOME-TRANSLOCATION; SARCOMA TRANSLOCATION; CHIMERIC TRANSCRIPTS; INSITU HYBRIDIZATION;
Keywords:
Ewing's tumor; translocation (10;11;22); complex translocation; fluorescence in situ hybridization;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Life Sciences
Citazioni:
54
Recensione:
Indirizzi per estratti:
Indirizzo: Noguera, R Univ Valencia, Sch Med, Dept Pathol, Avda Blasco Ibanez 17, Valencia 46010, Spain Univ Valencia Avda Blasco Ibanez 17 Valencia Spain 46010 Spain
Citazione:
R. Noguera et al., "Translocation (10;11;22)(p14;q24;q12) characterized by fluorescence in situ hybridization in a case of Ewing's tumor", DIAGN MOL P, 10(1), 2001, pp. 2-8

Abstract

It is well recognized that the identification by classic cytogenetics of t(11:22)(q24:q12) is a useful aid in the accurate diagnosis of Ewing's sarcoma and related tumors. This translocation induces the EWS/FLI-1 fusion transcript, which can be detected by reverse transcription-polymerase chain reaction. Recent studies have also used fluorescence in situ hybridization (FISH) to demonstrate the translocation. The authors coupled classic cytogenetics and FISH on tumor cells from the original specimen, the local recurrence, and the pulmonary metastasis as well as from the xenografted tumors in acase of extraosseous Ewing's sarcoma. FISH analysis not only confirmed thecytogenetic results but also allowed the identification of a tumor-specific chromosome change, consistent with a complex translocation, t(10:ll:22), as well as revealed other chromosomal rearrangements on both metaphases andinterphase nuclei of each material. In addition this technique served to identify, in the interphase nuclei of the original tumor, the clone that became dominant, from the cytogenetic point of view, in the lung metastasis and in the nude mice xenografted tumors. Current results indicate that the use of FISH on metaphases and interphase nuclei is an easy and reliable approach to complement or even to substitute classic cytogenetic studies for thedetection of specific chromosomal rearrangements, especially for determining complex translocations and for describing tumoral clones with different cytogenetic markers.

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Documento generato il 01/04/20 alle ore 20:37:00