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Titolo:
Pancreas development in zebrafish: Early dispersed appearance of endocrinehormone expressing cells and their convergence to form the definitive islet
Autore:
Biemar, F; Argenton, F; Schmidtke, R; Epperlein, S; Peers, B; Driever, W;
Indirizzi:
Univ Freiburg, Inst Biol 1, Abt Entwicklungsbiol, D-79104 Freiburg, Germany Univ Freiburg Freiburg Germany D-79104 gsbiol, D-79104 Freiburg, Germany Univ Padua, Dipartimento Biol, I-35131 Padua, Italy Univ Padua Padua Italy I-35131 , Dipartimento Biol, I-35131 Padua, Italy Univ Liege, Inst Chim, Lab Biol Mol & Genie Genet, B-4000 Sart Tilman, Belgium Univ Liege Sart Tilman Belgium B-4000 Genet, B-4000 Sart Tilman, Belgium
Titolo Testata:
DEVELOPMENTAL BIOLOGY
fascicolo: 2, volume: 230, anno: 2001,
pagine: 189 - 203
SICI:
0012-1606(20010215)230:2<189:PDIZED>2.0.ZU;2-H
Fonte:
ISI
Lingua:
ENG
Soggetto:
ONE-EYED PINHEAD; BETA-CELLS; TRANSCRIPTION FACTOR; ENDODERM FORMATION; HOMEOBOX GENE; DANIO-RERIO; NO TAIL; HOMEODOMAIN PROTEIN; MOUSE PANCREAS; MICE LACKING;
Keywords:
zebrafish; pancreas; endoderm; insulin;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
83
Recensione:
Indirizzi per estratti:
Indirizzo: Driever, W Univ Freiburg, Inst Biol 1, Abt Entwicklungsbiol, Hauptstr 1, D-79104 Freiburg, Germany Univ Freiburg Hauptstr 1 Freiburg Germany D-79104urg, Germany
Citazione:
F. Biemar et al., "Pancreas development in zebrafish: Early dispersed appearance of endocrinehormone expressing cells and their convergence to form the definitive islet", DEVELOP BIO, 230(2), 2001, pp. 189-203

Abstract

To begin to understand pancreas development and the control of endocrine lineage formation in zebrafish, we have examined the expression pattern of several genes shown to act in vertebrate pancreatic development: pdx-1, insulin (W. M. Milewski et al., 1998, Endocrinology 139, 1440-1449), glucagon, somatostatin (E. Argenton et al., 1999, Mech. Dev. 87, 217-221), islet-1 (Korzh et al., 1993, Development 118, 417-425), nkx2.2 (Barth and Wilson, 1995, Development 121, 1755-1768), and pax6.2 (Nornes et al., 1998, Mech. Dev.77, 185-196). To determine the spatial relationship between the exocrine and the endocrine compartments, we have cloned the zebrafish trypsin gene, adigestive enzyme expressed in differentiated pancreatic exocrine cells. Wefound expression of all these genes in the developing pancreas throughout organogenesis. Endocrine cells first appear in a scattered fashion in two bilateral rows close to the midline during mid-somitogenesis and converge during late-somitogenesis to form a single islet dorsal to the nascent duodenum. We have examined development of the endocrine lineage in a number of previously described zebrafish mutations. Deletion of chordamesoderm in floating head (Xnot homolog) mutants reduces islet formation to small remnants, but does not delete the pancreas, indicating that notochord is involved in proper pancreas development, but not required for differentiation of pancreatic cell fates. In the absence of knypek gene function, which is involved in convergence movements, the bilateral endocrine primordia do not merge. Presence of trunk paraxial mesoderm also appears to be instrumental for convergence since the bilateral endocrine primordia do not merge in spadetail mutants. We discuss our findings on zebrafish pancreatogenesis in the light of evolution of the pancreas in chordates. (C) 2001 Academic Press.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 06/04/20 alle ore 04:41:05