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Titolo:
Milk and blood as alternative sources of mRNA for studies of genetic polymorphism of alpha S1 casein in goats
Autore:
Tokarska, M; Szumska, D; Kosowska, B; Dobosz, T; Zdrojewicz, Z;
Indirizzi:
Univ Agr, Dept Anim Breeding & Genet, PL-51631 Wroclaw, Poland Univ Agr Wroclaw Poland PL-51631 eding & Genet, PL-51631 Wroclaw, Poland Med Acad, Dept Forens Med, Wroclaw, Poland Med Acad Wroclaw PolandMed Acad, Dept Forens Med, Wroclaw, Poland Med Acad, Inst Forens Med, Wroclaw, Poland Med Acad Wroclaw PolandMed Acad, Inst Forens Med, Wroclaw, Poland Med Univ, Dept Endocrinol & Diabetol, Wroclaw, Poland Med Univ Wroclaw Poland iv, Dept Endocrinol & Diabetol, Wroclaw, Poland Med Univ, Clin Endocrinol & Diabetol, Wroclaw, Poland Med Univ Wroclaw Poland iv, Clin Endocrinol & Diabetol, Wroclaw, Poland
Titolo Testata:
CZECH JOURNAL OF ANIMAL SCIENCE
fascicolo: 1, volume: 46, anno: 2001,
pagine: 17 - 21
SICI:
1212-1819(200101)46:1<17:MABAAS>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
ALPHA(S1)-CASEIN LOCUS; SAANEN BREEDS; ALPINE; BUCKS;
Keywords:
polymorphism; alpha S1 casein; cDNA; goats; PCR;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Citazioni:
14
Recensione:
Indirizzi per estratti:
Indirizzo: Tokarska, M Univ Agr, Dept Anim Breeding & Genet, Ul Kozuchowska 7, PL-51631 Wroclaw, Poland Univ Agr Ul Kozuchowska 7 Wroclaw Poland PL-51631 law, Poland
Citazione:
M. Tokarska et al., "Milk and blood as alternative sources of mRNA for studies of genetic polymorphism of alpha S1 casein in goats", CZEC J ANIM, 46(1), 2001, pp. 17-21

Abstract

Caprine alpha S1 casein gene polymorphism is responsible for quality of main processing parameters of milk, related with casein and protein content, processing quality and cheese flavour. The main 7 alleles are divided into 4 groups: strong alleles (which code 3.6 g/l), medium allele (1.6 g/l), weak alleles (0.6 g/l) and null allele, associated with lack of alpha S1 casein in milk. Milk mononuclear cells (MNC) and peripheral blood mononuclear cells (PBMC) of 16 goats were used as a source of mRNA of alpha S1 casein gene. Complementary DNA (cDNA) was obtained from both, milk and blood sources using RT-PCR. Specific primers allowed to distinguish strong alleles (A, B,C) from weak alleles (D, E) and medium allele (E), since allele F has 111 bp deletion (exons 9-11), allele D deletion of 33 bp (exon 9) and allele E is associated with 457 bp insertion in exon 19. identical results obtained using both, somatic cells of milk and peripheral blood leukocytes indicate that they may be used alternatively as mRNA sources for genetic polymorphism analyses of alpha S1 casein gene in goats. The disadvantage was a failureto isolate mRNA from either milk or blood collected in June-October.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 28/09/20 alle ore 15:11:57