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Titolo:
Nitric oxide induces apoptosis in the fat body cell line IPLB-LdFB from the insect Lymantria dispar
Autore:
Ottaviani, E; Barbieri, D; Malagoli, D; Franchini, A;
Indirizzi:
Univ Modena & Reggio Emilia, Dept Anim Biol, I-41100 Modena, Italy Univ Modena & Reggio Emilia Modena Italy I-41100 , I-41100 Modena, Italy
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
fascicolo: 2, volume: 128, anno: 2001,
pagine: 247 - 254
SICI:
1096-4959(200102)128:2<247:NOIAIT>2.0.ZU;2-6
Fonte:
ISI
Lingua:
ENG
Soggetto:
SMOOTH-MUSCLE CELLS; SYNTHASE ACTIVITY; IN-VITRO; PURIFICATION; EXPRESSION; ENDOTOXIN; FLAVOPROTEIN; MACROPHAGES; INHIBITION; MECHANISM;
Keywords:
insect cell line (IPLB-LdFB); nitric oxide; NO synthase; L-NAME; 1400W; apoptosis; sodium nitroprusside; N-acetyl-L-cysteine;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
40
Recensione:
Indirizzi per estratti:
Indirizzo: Ottaviani, E Univ Modena & Reggio Emilia, Dept Anim Biol, Via Campi 213-D,I-41100 Modena, Italy Univ Modena & Reggio Emilia Via Campi 213-D Modena Italy I-41100
Citazione:
E. Ottaviani et al., "Nitric oxide induces apoptosis in the fat body cell line IPLB-LdFB from the insect Lymantria dispar", COMP BIOC B, 128(2), 2001, pp. 247-254

Abstract

The presence of immunoreactive inducible nitric oxide synthase molecules (ir-iNOS) is demonstrated in the Lymantria dispar IPLB-LdFB cell lint. The maximum ir-iNOS inducibility is observed 18 h after incubation with sodium nitroprusside (SNP). The increase in NO provoked by SNP in turn induces apoptosis. However, this phenomenon is observed only after 48 h. The NOS-inhibitors N-omega-nitro-L-arginine methyl ester (L-NAME) and N-[3-(aminomethyl)-benzyl]acetamide (1400W) were both unable to block the SNP-induced apoptosis at all the concentrations used. Incubation with SNP plus N-acetyl-L-cysteine (NAC) further augmented the percentage of cell death with respect to SNP used alone, and this process is seen earlier, i.e. after 24 h. Moreover, the induction of apoptosis in the presence of NAC is time- and concentration-dependent. The high percentage of cell death with SNP + NAC suggests thatNAC forms S-nitrosothiols with NO, resulting in an increase in the bioavailability of NO. In conclusion, these findings show the existence of a closerelationship between mammalian and invertebrate cells with regards to SNP and NAC induction and the related NO response. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 03/04/20 alle ore 08:01:59