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Titolo:
The molecular basis of O-2-sensing and hypoxia tolerance in pheochromocytoma cells
Autore:
Conrad, PW; Conforti, L; Kobayashi, S; Beitner-Johnson, D; Rust, RT; Yuan, Y; Kim, HW; Kim, RH; Seta, K; Millhorn, DE;
Indirizzi:
Univ Cincinnati, Coll Med, Dept Mol & Cellular Physiol, Cincinnati, OH 45267 USA Univ Cincinnati Cincinnati OH USA 45267 Physiol, Cincinnati, OH 45267 USA
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
fascicolo: 2, volume: 128, anno: 2001,
pagine: 187 - 204
SICI:
1096-4959(200102)128:2<187:TMBOOA>2.0.ZU;2-B
Fonte:
ISI
Lingua:
ENG
Soggetto:
ACTIVATED PROTEIN-KINASE; TYROSINE-HYDROXYLASE GENE; RAT PC12 CELLS; PULMONARY-ARTERY MYOCYTES; PAS DOMAIN PROTEIN-1; GATED K+ CHANNELS; TRANSCRIPTION FACTOR; INDUCIBLE FACTOR-1-ALPHA; ADENOSINE RECEPTORS; SIGNALING PATHWAYS;
Keywords:
gene expression; signal transduction; transcription; adenosine; pheochromocytoma; PC12 cells; mitogen-activated protein kinase (MAPK); p38; EPAS1;
Tipo documento:
Review
Natura:
Periodico
Settore Disciplinare:
Life Sciences
Citazioni:
62
Recensione:
Indirizzi per estratti:
Indirizzo: Millhorn, DE Univ Cincinnati, Coll Med, Dept Mol & Cellular Physiol, POB 67-0576, Cincinnati, OH 45267 USA Univ Cincinnati POB 67-0576 Cincinnati OH USA 45267 45267 USA
Citazione:
P.W. Conrad et al., "The molecular basis of O-2-sensing and hypoxia tolerance in pheochromocytoma cells", COMP BIOC B, 128(2), 2001, pp. 187-204

Abstract

Hypoxia is a common environmental stimulus. However, very little is known about the mechanisms by which cells sense and respond to changes in oxygen. Our laboratory has utilized the PC12 cell line in order to study the biophysical and molecular response to hypoxia, The current review summarizes ourresults. We demonstrate that the O-2-sensitive K+ channel, Kv1.2, is present in PC12 cells and plays a critical role in the hypoxia-induced depolarization of PC12 cells. Previous studies have shown that PC12 cells secrete a variety of autocrine/paracrine factors, including dopamine, norepinephrine,and adenosine during hypoxia. We investigated the mechanisms by which adenosine modulates cell function and the effect of chronic hypoxia on this modulation. Finally, we present results identifying the mitogen- and stress-activated protein kinases (MAPKs and SAPKs) as hypoxia-regulated protein kinases. Specifically, we show that p38 and an isoform, p38 gamma, are activated by hypoxia. In addition, our results demonstrate that the p42/p44 MAPK protein kinases are activated by hypoxia. We further show that p42/p44 MAPK is critical for the hypoxia-induced transactivation of endothelial PAS-domain protein 1 (EPAS1), a hypoxia-inducible transcription factor. Together, these results provide greater insight into the mechanisms by which cells sense and adapt to hypoxia. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 23/01/20 alle ore 03:51:08