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Titolo:
Comparison of in vitro systems of protein digestion using either mammal orfish proteolytic enzymes
Autore:
Moyano, FJ; Savoie, L;
Indirizzi:
Univ Almeria, Escuela Politecn Super, Departo Biol Aplicada, Almeria 04120, Spain Univ Almeria Almeria Spain 04120 rto Biol Aplicada, Almeria 04120, Spain Univ Laval, Fac Sci Agr & Alimentat, Dept Nutr Humaine & Consommat, QuebecCity, PQ G1K 7P4, Canada Univ Laval Quebec City PQ Canada G1K 7P4 , QuebecCity, PQ G1K 7P4, Canada
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY
fascicolo: 2, volume: 128, anno: 2001,
pagine: 359 - 368
SICI:
1095-6433(200102)128:2<359:COIVSO>2.0.ZU;2-O
Fonte:
ISI
Lingua:
ENG
Soggetto:
TROUT ONCORHYNCHUS-MYKISS; SALMO-GAIRDNERI; INVITRO METHOD; DIGESTIBILITY; PROTEASES; ASSAY; ABSORPTION;
Keywords:
fish proteases; in vitro assays; mammal proteases; protein digestibility;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
27
Recensione:
Indirizzi per estratti:
Indirizzo: Moyano, FJ Univ Almeria, Escuela Politecn Super, Departo Biol Aplicada, Almeria 04120, Spain Univ Almeria Almeria Spain 04120 licada, Almeria 04120, Spain
Citazione:
F.J. Moyano e L. Savoie, "Comparison of in vitro systems of protein digestion using either mammal orfish proteolytic enzymes", COMP BIOC A, 128(2), 2001, pp. 359-368

Abstract

Hydrolysis of three different proteins by either crude fish digestive extracts or purified mammal proteases was assayed using two different in vitro systems. The closed system was a modification of the pH-stat method including a previous acid digestion. The open system used a digestion cell containing a semi-permeable membrane which allowed continuous separation of the final products of hydrolysis with a molecular cut-off of 1000 Da. Assays in both systems resulted a similar arrangement of the tested proteins in relation to their ability to be hydrolyzed, with casein > fish meal greater than or equal to soybean meal. With the exception of casein, no significant differences were found between results produced by any of the enzyme sources using the closed system. In constrast, significantly higher hydrolysis of allproteins was produced by mammal enzymes under conditions operating in the open system. Differences in the rate of release of amino acids measured in this latter system were related both to the type of protein and the origin of the enzymes. When using purified mammal enzymes, release of lysine or phenylalanine from casein and soybean was high, but low from fishmeal, Isoleucine and valine present in fishmeal were preferentially hydrolyzed by commercial enzymes, but glycine and proline by fish enzymes. (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 09/07/20 alle ore 19:41:53