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Titolo:
Induction of 7-ethoxyresorufin-o-deethylase activity by planar chlorinatedhydrocarbons and polycyclic aromatic hydrocarbons in cell lines from the rainbow trout pituitary
Autore:
Tom, DJ; Lee, LEJ; Lew, J; Bols, NC;
Indirizzi:
Univ Waterloo, Dept Biol, Waterloo, ON N2L 3G1, Canada Univ Waterloo Waterloo ON Canada N2L 3G1 ol, Waterloo, ON N2L 3G1, Canada Univ Saskatchewan, Dept Vet Anat, Saskatoon, SK S7N 5B4, Canada Univ Saskatchewan Saskatoon SK Canada S7N 5B4 skatoon, SK S7N 5B4, Canada
Titolo Testata:
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY A-MOLECULAR AND INTEGRATIVE PHYSIOLOGY
fascicolo: 2, volume: 128, anno: 2001,
pagine: 185 - 198
SICI:
1095-6433(200102)128:2<185:IO7ABP>2.0.ZU;2-T
Fonte:
ISI
Lingua:
ENG
Soggetto:
POLYCHLORINATED-BIPHENYLS PCBS; TOXIC EQUIVALENT FACTORS; CYP1A1 GENE-EXPRESSION; AH RECEPTOR; ALPHA-NAPHTHOFLAVONE; BETA-NAPHTHOFLAVONE; FACTORS TEFS; CYTOCHROME-P450 1A1; RELATIVE POTENCIES; DIOXIN RECEPTOR;
Keywords:
rainbow trout; pituitary; cell lines; EROD; CYP1A; dioxin; PCB;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Agriculture,Biology & Environmental Sciences
Life Sciences
Citazioni:
45
Recensione:
Indirizzi per estratti:
Indirizzo: Bols, NC Univ Waterloo, Dept Biol, Waterloo, ON N2L 3G1, Canada Univ Waterloo Waterloo ON Canada N2L 3G1 loo, ON N2L 3G1, Canada
Citazione:
D.J. Tom et al., "Induction of 7-ethoxyresorufin-o-deethylase activity by planar chlorinatedhydrocarbons and polycyclic aromatic hydrocarbons in cell lines from the rainbow trout pituitary", COMP BIOC A, 128(2), 2001, pp. 185-198

Abstract

The induction of 7-ethoxyresorufin-o-deethylase (EROD) activity was examined in three rainbow trout pituitary cell lines: RTP-91E, RTP-91F and RTP-2. RTP-91E and RTP-91F were developed from the pituitary of a male and have epithelial-like and fibroblast-Like morphologies, respectively. RTP-2, whichwas described previously, was developed from the pituitary of a female andhas an epithelial-like shape. In all cell lines EROD activity was induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Immunoblotting with the polyclonal antibody, anti-trout CYP1A1(277-294)/KLH, confirmed induction of a 58-kDa polypeptide, Potential inhibitors of the aryl hydrocarbon receptor, geldanamycin and alpha -naphthoflavone, inhibited EROD induction by TCDD. Other compounds inducing EROD activity were 1,2,3,7,8-pentachlorodibenzo-p-dioxin (PCDD), 2,3,7,8-tetrachlorodibenzofuran (TCDF), 3,3',4,4',5-pentachlorobiphenyl (PCB 126), and 3-methylcholanthrene (3MC). When judged by the concentration eliciting 50% of the maximal response (EC50)induction was similar in RTP-2 and RTP-91E, and Less effective in RTP-91F. Regardless of the cell Line, the rank order from most to least potent inducer on the basis of EC50 value was TCDD greater than or equal to PCDD > TCDF > PCB 126 much greater than 3MC, When induction potencies were expressed relative to TCDD, thevalues obtained with the pituitary cell lines were similar to previously published values derived with a rainbow trout liver cell line, (C) 2001 Elsevier Science Inc. All rights reserved.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 26/11/20 alle ore 11:48:37