Catalogo Articoli (Spogli Riviste)

OPAC HELP

Titolo:
Cloning and characterization of a novel gene, DRH1, down-regulated in advanced human hepatocellular carcinoma
Autore:
Yamamoto, Y; Sakamoto, M; Fujii, G; Kanetaka, K; Asaka, M; Hirohashi, S;
Indirizzi:
Natl Inst Canc Res, Div Pathol, Chuo Ku, Tokyo 1040045, Japan Natl Inst Canc Res Tokyo Japan 1040045 ol, Chuo Ku, Tokyo 1040045, Japan Hokkaido Univ, Fac Med, Dept Internal Med 3, Sapporo, Hokkaido 0608038, Japan Hokkaido Univ Sapporo Hokkaido Japan 0608038 oro, Hokkaido 0608038, Japan
Titolo Testata:
CLINICAL CANCER RESEARCH
fascicolo: 2, volume: 7, anno: 2001,
pagine: 297 - 303
SICI:
1078-0432(200102)7:2<297:CACOAN>2.0.ZU;2-E
Fonte:
ISI
Lingua:
ENG
Soggetto:
COMPARATIVE GENOMIC HYBRIDIZATION; ALPHA-FETOPROTEIN LEVELS; NUCLEAR EXPORT SIGNAL; DIFFERENTIAL DISPLAY; MESSENGER-RNA; RETINOBLASTOMA PROTEIN; BREAST-CANCER; EXPRESSION; CDNA; PROGRESSION;
Tipo documento:
Article
Natura:
Periodico
Settore Disciplinare:
Clinical Medicine
Citazioni:
43
Recensione:
Indirizzi per estratti:
Indirizzo: Hirohashi, S Natl Inst Canc Res, Div Pathol, Chuo Ku, 5-1-1 Tsukiji, Tokyo1040045, Japan Natl Inst Canc Res 5-1-1 Tsukiji Tokyo Japan 1040045 , Japan
Citazione:
Y. Yamamoto et al., "Cloning and characterization of a novel gene, DRH1, down-regulated in advanced human hepatocellular carcinoma", CLIN CANC R, 7(2), 2001, pp. 297-303

Abstract

Few genes related to carcinogenesis and progression of hepatocellular carcinoma (HCC) have been identified to date. In the present study, we report the cloning and characterization of a novel gene, DRH1, which is frequently down-regulated in HCC. The full-length DRH1 clone contains an open reading frame of 1257 nucleotides encoding 419 amino acids. The deduced DRH1 protein shows 41% identity to VDUP1, expression of which is rapidly induced by 1,25-dihgdroxyvitamin D-3. The DRH1 gene was localized to chromosome 15, and DRH1 protein was mainly observed in the cytoplasm of transiently transfected cells. Real-time quantitative reverse transcription-PCR analysis showed that the expression level of DRH1 was reduced in 29 of 35 (83 %) HCCs compared with corresponding noncancerous liver tissue. The average (mean +/- SE) ratio of DRH1 expression level in tumor to corresponding noncancerous tissue was significantly different between well, moderately, and poorly differentiated HCCs (1.15 +/- 0.23, 0.69 +/- 0.10, and 0.19 +/- 0.04, respectively)and between HCCs without and with vascular invasion (0.93 +/- 0.16 and 0.46 +/- 0.07, respectively). These results indicate that the down-regulation of DRH1 occurs not at an early stage but rather at a late stage of HCC progression. Although the function of DRH1 protein is still unknown, our findings suggest that DRH1 is related to the progression of HCC and may provide anew prognostic factor.

ASDD Area Sistemi Dipartimentali e Documentali, Università di Bologna, Catalogo delle riviste ed altri periodici
Documento generato il 07/07/20 alle ore 21:16:51